SCREENING OF ACTIVE COMPOUNDS FROM Artemisia annua USING HFC-134A SUBCRITIC EXTRACTION SYSTEM
Artemisia annua is known as the source of artemisinin, a sesqueterpene lactone possessing endoperoxide moeity with antiplasmodium activity. Sub-critical extraction of artemisinin from Artemisia annua using R134a solvent was conducted. A close system cycle process was constructed to investigate the ability of R134a to extract artemisinin together with other active compounds. Artemisinin and other isolated compouds yield was investigated at 30°C and presure process at 10 barr, with variation of process time. The extracted product was analysed as artemisinin as the target compounds with several isolated compounds as by product. Artemisinin content was reported ranging up to 0,5%, and the sub critic system was reported to be more efficient and effective comparing to other conventional extraction methods. The phytonics HFC-134a sub critics process can be used for extraction in selected pharmacologically active products from herbal plants.
Bhakuni R. S., D. C. Jain, R. P. Sharma and S. Kumar 2001 Secondary metabolites of Artemisia annua and their biological activity. CURRENT SCIENCE, VOL. 80, NO. 1 pp 35-48
Corr. S (2002). 1,1,1,2-Tetrafluoroethane; from refrigerant and propellant to solvent. Journal of Fluorine Chemistry 118: 55-57
Emmen H. H., E. M. G. Hoogendijk, W. A. A. Klöpping-Ketelaars, H. Muijser, E. Duistermaat,J. C. Ravensberg, D. J. Alexander, D. Borkhataria, G. M. Rusch and B. Schmit, Regul. Toxicol. Pharm., 2000, 32, 22–35 (human safety and pharmacokinetics of HFC 134a)
Lapkin AA1, Plucinski PK, Cutler M. 2006. Comparative assessment of technologies for extraction of artemisinin. J Nat Prod. 2006 Nov;69(11):1653-64.
Louli, V. N. Ragoussis, K. Magoulas, Recovery of phenolic antioxidants from wine industry by-products, Bioresour. Technol. 92 (2004) 201–208.
Moure, J.M. Cruz, D. Franco, J.M. Domínguez, J. Sineiro, H. Domínguez, et al., Natural antioxidants from residual sources, Food Chem. 72 (2001) 145–171.
Pessoa A. S., R. Podestá, J.M. Block, E. Franceschi, C. Dariva, M. Lanza, Extraction of pequi (Caryocar coriaceum) pulp oil using subcritical propane: Determination of process yield and fatty acid profile. J. Supercrit. Fluids, 101 (2015) 95-103.
Souhila Laboukhi-Khorsi, Kamel Daoud, and Smain Chemat (2017) Efficient Solvent Selection Approach for High Solubility of Active Phytochemicals: Application for the Extraction of an Antimalarial Compound from Medicinal Plants. ACS Sustainable Chem. Eng., 2017, 5 (5), pp 4332–4339. DOI: 10.1021/acssuschemeng.7b00384
Sunil Kumar, 2014, Design And Development Of Extraction Process In The Isolation Of Phytopharmaceuticals From Plant Sources. International Journal of Medicine and Health Profession Research. 1(1), 28 - 38.
Wilde P.F., T.J. Noakes, R.L. Powell, (2001); US Patent 6224847 J.E Haworth, WO 0126472
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.