APHRODISIAC ACTIVITY OF ETHANOL EXTRACT OF Cratoxylum sumatranum (JACK) BLUME STEMS ON ISOLATED RAT CORPUS CAVERNOSUM

  • Sjarif Ismail
  • Meiliati Aminyoto Faculty of Medicine Mulawarman University, Samarinda
Keywords: Cratoxylum sumatranum, aphrodisiac, in vitro, corpus cavernosum, vasodilation

Abstract

Cratoxylum sumatranum (Jack) Blume of the Hypericaceae family is known as “Bentaleng†by Dayak Benuaq. In ethnobotany, Cratoxylum sumatranum stems (CSS) is used as energy drink or aphrodisiac, but its effect has not been scientifically proven. Research objective to study the aphrodisiac activity of CSS extract by screening the aphrodisiac activity in vitro. Method: CSS was collected from Kutai Kertanegara Regency, East Kalimantan Province. Extraction was by maceration with ethanol solvent for three days. Re-maceration was done twice. In vitro screening of aphrodisiac activity used isolated rat corpus cavernosum. The organ was placed into a 10 mL chamber containing Krebs-Henselheit solution at pH 7.4, 37°C and aerated with carbogen gas. After acclimation, a contraction test was performed with phenylephrine solution and after reaching the peak of contraction at plateau the Control (solvent extract) or CSS ethanol extract was administerd at cumulatively increased concentration. Vasodilation activity was known if the contraction response was decreased after the extract’s administration and expressed in percent contraction with negative value. Results: CSS ethanol extract induce vasodilatory response on rat Corpus cavernosum blood vessels. Vasodilation activity is increasing with increasing concentration of extract given compared to Control. Conclusion the aphrodisiac activity of CSS ethanol extracts is directly through the vasodilation action mechanism on blood vessels in the rat corpus cavernosum.  

Downloads

Download data is not yet available.

References

[1] NIH Concensus Development Panel on Impotence. 1993. Impotence. Journal of the American Medical Association, 270:83-90.
[2] Prins J, Blanker MH, Bohnen AM, Thomas S, & Bosch JLHR. 2002. Prevalence erectile dysfunction: a systematic review of population-based studies. International Journal of Impotence Research, 14:422-432.
[3] Technavio. 2016. Global erection dysfunction market 2016-2020. SKU: IRTNTR9506, Inviniti Research Limited. Available at: https://www.technavio.com/report/global-health-and-wellness-global-erectile-dysfunction-market-2016-2020. Acessed 10/23/2017.
[4] Grand View Research. 2017. Erectile dysfunction drug market worth $ 3.2 billion by 2022. Grand View Research Inc. Available at: http://www.grandviewresearch.com/press-release/global-erectile-dysfunction-drugs-market. Acessed 10/23/2017.
[5] The Plant List. 2017. Cratoxylum sumatranum (Jack) Blume. The plant list: a working list of all plant species. Available at: http://www.theplantlist.org/tpl1.1/record/kew-2742440. Acessed 10/23/2017.
[6] Heyne K. 1987. Tumbuhan berguna Indonesia. Diterjemahkan oleh Badan Litbang Kehutanan Jakarta. Penerbit: Koperasi Karyawan Departemen Kehutanan. Page 1371-1373.
[7] Badan Penelitian dan Pengembangan Kalimantan Timur. 2006. Identifikasi dan pengembangan fitokimia tanaman obat unggulan Kaltim. Bidang Ekonomi dan Pembangunan Badan Penelitian dan Pengembangan Provinsi Kaltim. Laporan Penelitian.
[8] Keegan A, Cotter MA, & Cameron NE. 1999. Effects of chelator treatment on aorta and corpus cavernosum from diabetic rats. Free Radical Biology and Medicine Journal, 27(5-6):536-43.
[9] Paskaloglu K, Sener G, & Ayanğolu-Dülger G. 2004. Melatonin treatment protects against diabetes-induced functional and biochemical changes in rat aorta and corpus cavernosum. European Journal of Pharmacology, 499(3):345-54.
[10] Anderson KE. 2011. Mechanisms of penile erection and basis for pharmacological treatment of erectile dysfunction. Pharmacological Review, 63:811-859.
[11] Lue TF. 2000. Erectile dysfunction. The New England Journal of Medicine, 342(24)1802–1813.
[12] Burnett AL. 2006. The role of nitric oxide in erectile dysfunction: implications for medical therapy. Journal of Clinical Hypertension, 8(12):53–62.
[13] Burnett AL, Ricker DD, Chamness SL, Maguire MP, Crone JK, Bredt DS, Snyder SH, & Chang TS. 1995. Localization of nitric oxide synthase in the reproductive organs of the male rat. Biology of Reproduction, 52(1):1–7.
[14] Gonzalez CM, Brannigan RE, Bervig T, Zelner D, Podlasek CA, McKenna KE, & McVary KT. 2001. Protein and gene expression of nitric oxide synthase isoforms I and III in the rat penile shaft. Journal of Andrology, 22(1):54–61, 2001.
[15] Cartledge JS, Minhas I, Eardley, & Morrison JF. 2000. Endothelial and neuronal derived nitric oxide mediated relaxation of corpus cavemosal smooth muscle in a new rat, in vitro model of erectile function. International Journal of Impotence Research,12:213-221.
Published
2018-07-26