α-Glucosidase Inhibitory Activity of In Vitro Combination of 96% Ethanolic Extract of Brotowali Stem (Tinospora cordifolia) and Cashew Apple (Anacardium occidentale L.)
Cashew tree (Anacardium occidentale L), contains flavonoid compound which plays a role in the medical treatment of Diabetes Mellitus because this compound has activity as an α-glucosidase inhibitor. Brotowali stem extract (Tinospora cordfolia) demonstrated α-glucosidase inhibitory (AGIS) activity. This research aimed to measure 96% ethanolic extract AGIS activity of brotowali-cashew apple combination (BJM). The research was conducted by using ELISA reader instrument at 405 nm for AGIS activity test. The IC50 was calculated from each sample. The IC50 of brotowali stem and cashew apple was 34,61 μg/ml and 109,32 μg/ml. Then, the combination of brotowali and cashew apple extract was mixed with each concentration of 0,5%. From 1:0, 1:2, 1:1, 2:1, and 0:1 BJM comparison resulted in AGIS power percentage averages of 84.05%, 69.51%, 70.92%, 68.10%, 40.37%. The results of this research indicated that brotowali stem has the potential as AGIS, while cashew apple has not any potential towards AGIS and that extract combination does not give synergic effect. Therefore, the use of a combination of brotowali-cashew apple extract is not as effective as the use of brotowali single extracts to inhibit the α-glucosidase enzyme
World Health Organization, 2016. Global report on diabetes. World Health Organization.
Kalra, S., 2014. Alpha glucosidase inhibitors. JPMA. The Journal of the Pakistan Medical Association. 64. 474-476.
Kwon, Y.-I., Apostolidis, E., Shetty, K., 2008. In vitro studies of eggplant (Solanum melongena) phenolics as inhibitors of key enzymes relevant for type 2 diabetes and hypertension. Bioresource Technology. 99. 2981-2988.
Campbell, L. K., Baker, D. E., Campbell, R. K., 2000. Miglitol: assessment of its role in the treatment of patients with diabetes mellitus. Annals of Pharmacotherapy. 34. 1291-1301.
Patel, M. B., Mishra, S., 2011. Hypoglycemic activity of alkaloidal fraction of Tinospora cordifolia. Phytomedicine. 18. 1045-1052.
Chougale, A. D., Ghadyale, V. A., Panaskar, S. N., Arvindekar, A. U., 2009. Alpha glucosidase inhibition by stem extract of Tinospora cordifolia. Journal of Enzyme Inhibition and Medicinal Chemistry. 24. 998-1001.
Hamid, H., Yusoff, M., Liu, M., Karim, M., 2015. α-Glucosidase and α-amylase inhibitory constituents of Tinospora crispa: Isolation and chemical profile confirmation by ultra-high performance liquid chromatography-quadrupole time-of-flight/mass spectrometry. Journal of Functional Foods. 16. 74-80.
Trevisan, M., Pfundstein, B., Haubner, R., Würtele, G., Spiegelhalder, B., Bartsch, H., Owen, R., 2006. Characterization of alkyl phenols in cashew (Anacardium occidentale) products and assay of their antioxidant capacity. Food and Chemical Toxicology. 44. 188-197.
Alexander‐Lindo, R. L., Morrison, E. S. A., Nair, M. G., 2004. Hypoglycaemic effect of stigmast‐4‐en‐3‐one and its corresponding alcohol from the bark of Anacardium occidentale (cashew). Phytotherapy Research: An International Journal Devoted to Pharmacological and Toxicological Evaluation of Natural Product Derivatives. 18. 403-407.
Esimone, C., Okonta, J., Ezugwu, C., 2001. Blood sugar lowering effect of Anacardium occidentale leaf extract in experimental rabbit model. Journal of Natural Remedies. 1. 60-63.
de Brito, E. S., de Araújo, M. C. P., Lin, L.-Z., Harnly, J., 2007. Determination of the flavonoid components of cashew apple (Anacardium occidentale) by LC-DAD-ESI/MS. Food chemistry. 105. 1112-1118.
Wang, H., Du, Y.-J., Song, H.-C., 2010. α-Glucosidase and α-amylase inhibitory activities of guava leaves. Food chemistry. 123. 6-13.
Saifudin, A., 2014. Senyawa alam metabolit sekunder: Teori, konsep, dan teknik pemurnian. Deepublish.
Departemen Kesehatan Republik Indonesia, 2000. Parameter standar umum ekstrak tumbuhan obat. Jakarta: Departemen Kesehatan Republik Indonesia. 9-12.
Gandjar, I. G., Rohman, A., 2007. Kimia farmasi analisis. Pustaka Pelajar, Yogyakarta.
Sabu, M., Smitha, K., Kuttan, R., 2002. Anti-diabetic activity of green tea polyphenols and their role in reducing oxidative stress in experimental diabetes. Journal of ethnopharmacology. 83. 109-116.
Kim, Y.-M., Jeong, Y.-K., Wang, M.-H., Lee, W.-Y., Rhee, H.-I., 2005. Inhibitory effect of pine extract on α-glucosidase activity and postprandial hyperglycemia. Nutrition. 21. 756-761.
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