AKTIVITAS EKSTRAK ETANOL DAN FRAKSI AKAR SINGAWALANG (Petiveria alliacea L. ) TERHADAP JAMUR PENYEBAB KETOMBE DENGAN METODE BROTH MICRODILUTION

  • Niken Indriyanti
  • I Ketut Adnyana
  • Elin Yulinah Sukandar
Keywords: singalawang roots, olysulfide, Pityrosporum ovale

Abstract

Dandruff was an anomaly of scalp caused by abnormal growth of Pityrosporum ovale. Ketoconazole and sulfuric compounds known as antifungal, include antifungal against Pityrosporum ovale. One of medicinal plant that has polysulfide compounds was Singawalang (Petiveria alliacea L.). Activity of ethanol extract and fraction of singawalang roots tested using microdilution broth method appropriate to Clinical and Laboratory Standard Institute (CLSI) standard, then growth profiles determined by colony count. Microdilution test results showed that Singawalang roots extract has antifungal activity against Pityrosporum ovale with Minimum Inhibition Concentration (MIC) 16 μg/mL and Minimum Fungicidal Concentration (MFC) 64 μg/mL. Fraction that has highest activity against Pityrosporum ovale was n-hexane fraction of Singawalang roots with MIC 16 µg/ml dan MFC 128 μg/mL. The higher activity of the extract predicted that there were some polysulfide compounds have synergic activity.

 Key words : singalawang roots, polysulfide, Pityrosporum ovale

 

ABSTRAK

Ketombe adalah suatu keadaan anomali pada kulit kepala disebabkan jamur Pityrosporum ovale dalam jumlah diatas normal. Selama ini antijamur yang digunakan adalah ketokonazol. Selain itu, senyawa sulfur juga diketahui aktif terhadap jamur. Salah satu tanaman yang telah diteliti mengandung senyawa polisulfida adalah tanaman singawalang (Petiveria alliacea L.). Penelitian aktivitasnya terhadap Pityrosporum ovale dilakukan dengan Broth Microdilution sesuai standar Clinical and Laboratory Standard Institute (CLSI). Konsentrasi Hambat Minumum (KHM) terkecil ada pada ekstrak dan fraksi n-heksan, yaitu 16 μg/mL, seperempat dari aktivitas ketokonazol. Konsentrasi Fungisidal Minimum (KFM) terkecil ekstrak adalah 64 μg/mL, dan pada fraksi n-heksan ekstrak etanol akar singawalang dengan konsentrasi 128 μg/mL. Diduga aktivitas antijamur lebih kuat pada ekstrak karena adanya kombinasi aktivitas beberapa senyawa polisulfida yang bekerja sinergis.

Kata kunci : Akar singawalang, polisulfida, Pityrosporum ovale

Downloads

Download data is not yet available.

References

1.Benevides, J.P.C., Maria Claudia M.Y., Astrea M.G., Nidia F.R. dan V.S. Bolzani, 2000.: Antifungal Polysulphides from Petiveria alliacea,Journal of Phytochemistry, 57, 743–747.
2.Brooks, G.F., Butel J.S. dan Morse S.A , 2001.: Jawetz, Melnick &Adelberg’s
Medical Microbiology, McGraw Hill., 23th Edition.
3.CLSI ,2008.: M38-A2 Reference Method for Broth Dilution Antifungal Susceptibility Testing of Filamentous Fungi; Clinical and Laboratory Standard Institute, approved Standard-Second Edition.
4.Katzung B.G. , 2004.: Farmakologi Dasar dan Klinik, diterjemahkan oleh Bagian Farmakologi FK Unair, Buku 3 Ed. 8, Salemba Medika, 111-123.
5.Kim S., Kubec R. dan Musah R.A., , 2006.: Antibacterial and antifungal activity of sulfur-containing compounds from Petiveria alliacea, Journal of Ethnopharmacology,104, 188–192.
6.Ravichandran, G., Bharadwaj V.S., Kolhapure S.A. , 2004.: Evaluation of
the clinical efficacy and safety of ‘Anti-dandruff shampoo’ in the treatment of dandruff, ‘The Antiseptic’201 (1) 5-8.
7.Schwalbe, R., Moore L.S., Goodwin A.C., 2007.: Antimicrobial Susceptibility Testing Protocols, CRC Press Taylor & Francis Group, Broken Sound Parkway NW, 173-205, 220-228, 339-340.
8.Sukandar, E.Y., Suwendar. dan Ekawati E. , 2006. : Aktivitas ekstrak etanol herba seledri (Apium graveolens) dan daun urang aring (Eclipta prostata (L.)L.) terhadap Pityrosporum ovale, Majalah Farmasi Indonesia, 17(1), 7-12.
Published
2013-06-30
How to Cite
Indriyanti, N., Adnyana, I. K., & Sukandar, E. Y. (2013). AKTIVITAS EKSTRAK ETANOL DAN FRAKSI AKAR SINGAWALANG (Petiveria alliacea L. ) TERHADAP JAMUR PENYEBAB KETOMBE DENGAN METODE BROTH MICRODILUTION. Journal of Tropical Pharmacy and Chemistry, 2(2), 113-117. https://doi.org/10.25026/jtpc.v2i2.56