AKTIVITAS ANTIOKSIDAN EKSTRAK ETANOL DAUN BUAS-BUAS (Premna cordifolia Linn.) TERHADAP GAMBARAN HISTOPATOLOGI PARU TIKUS (Rattus norvegicus) WISTAR JANTAN PASCA PAPARAN ASAP ROKOK

  • Kristina Lusty Tohomi
  • Iswahyudi Iswahyudi
  • Sri Wahdaningsih
Keywords: Antioxidant, Premna cordifolia, Lung histopathology, Cigarette smoke

Abstract

Cigarette smoke is one of free radical source that can cause oxidative stress, emphysema, and inflamation on lung. Leaves of Premna cordifolia Linn. is one of antioxidant that can neutralize the free radical in body. The purpose of the research  is to find the antioxidant activity of Premna cordifolia leaves in reducing lung’s damage and to find the effective dose that can reduce lung’s damage after exposure of cigarette smoke. Dried leaves of Premna cordifolia are maserated with ethanol 70%.  Rats are divided into five groups, Group without treatment (CMC control), group that exposed to smoke, three groups that treated with 200 (P1), 400 (P2), and 600mg/kgBW (P3) of ethanolic extract of Premna cordifolia leaves after smoke exposure, and one group that treated with 18mg/kgBW of vitamin E as positive control after smoke exposure. All groups were treated with three cigarette without filter for 14 days. All rat were terminated on the fifteent day. Right lung were collected and subjected to necropsy and stained with Hematoksilin-Eosin. The result show that all doses of ethanolic extract of Premna cordifolia reduce lung damage in enlarge alveolar, thickening of alveolar’s wall, and infiltration of inflmation cells in rats. The effective dose is 600mg/kgbw because the potential of Premna cordifolia leaves extract was same with the potential of positive contol in reducing the lung damage.

Keywords: Antioxidant, Premna cordifolia, Lung histopathology, Cigarette smoke

 

ABSTRAK

Asap rokok merupakan salah satu sumber radikal bebas yang dapat menyebabkan stress oksidatif, emfisema dan peradangan paru. Daun buas-buas (Premna cordifolia Linn.) merupakan salah satu antioksidan yang dapat menetralisir keberadaan radikal bebas dalam tubuh. Tujuan dari penelitian ini adalah untuk mengetahui aktivitas antioksidan daun buas-buas dalam menurunkan derajat kerusakan paru pada tikus pasca paparan asap rokok dan dosis efektif daun buas-buas yang dapat menurunkan derajat kerusakan paru tikus akibat asap rokok. Simplisia daun buas-buas dimaserasi dengan etanol 70%. Tikus dibagi menjadi 5 perlakuan, yaitu kelompok  yang hanya diberi suspensi CMC (kontrol CMC), kelompok yang hanya diberi paparan asap rokok  (kontrol negatif), kelompok perlakuan P1, P2, P3 masing-masing diberi paparan asap rokok dan ekstrak etanol daun buas-buas dengan dosis 200, 400, serta dosis 600 mg/kgBB dan kelompok perlakuan kontrol Positif diberi paparan asap rokok dan suspensi vitamin E dosis 18 mg/kgBB. Semua kelompok diperlakukan setiap hari dengan menggunakan 3 batang rokok tanpa filter selama 14 hari . Kemudian pada hari ke 15 semua tikus diterminasi dan diambil paru sebelah kanan untuk selanjutnya dibuat preparat histopatologi menggunakan pewarnaan Hematoksilin-Eosin. Hasil penelitian menunjukkan bahwa ekstrak etanol daun buas-buas dapat menurunkan tingkat kerusakan paru-paru pada pelebaran lumen alveolus, penebalan dinding alveolus dan infiltrasi sel radang pada tikus yang terpapar asap rokok. Dosis ekstrak etanol daun buas-buas dosis 600 mg/kgBB merupakan dosis efektif untuk menurunkan tingkat kerusakan paru-paru hingga tidak berbeda signifikan (p>0,05) dibandingkan dengan kontrol positif.

Kata kunci: Premna cordifolia, Antioksidan, Histopatologi paru, Asap rokok

Downloads

Download data is not yet available.

References

1.Abdelmohsen Usama Ramadan, Matthias Szesny, Eman Maher Othman, TanjaSchirmeister, Stephanie Grond, Helga Stopper and Ute Hentschel. Antioxidant and Anti-Protease Activities of Diazepinomicin from the Sponge-Associated Micromonospora Strain RV115. Mar. Drugs. 2012. ISSN : 1660-3397. 10 : 2208-2221.
2.Bakar, F. A, Mohamed, S, Hamid, A. A, dan Mustafa, R. A. Total Phenolic Compounds, Flavonoids, And Radical Scavenging Activity Of 21 Selected Tropical Plants. Journal of Food Science.Januari-Februari 2010; 75(1): Hal 28-35.
3.Büyükbas S, Kürsat U, Elif D, Kemal B. Oxidarive Stress and Antioxidant Status in Bronchoalveolar Lavage Fluid, Plasma and Erythrocyte of Critically Mixed Ill With Respiratory Failure. Eur J Gen Med.2008; 5(3):140-146.
4.Comalada M. Inhibition of pro-inflammatory markers in primary bone marrow-derived mouse macrophages by naturally occurring flavonoids: Analysis of the structure–activity relationship. Biochemical pharmacology. 2006: (72)1010–1021.
5.Departemen Kesehatan Republik
Indonesia. Parameter Standar Umum Ekstrak Tumbuhan Obat. Jakarta: Departemen Kesehatan Republik Indonesia; 2000. Hal 5, 9-12.
6.Dietrich M, Gladys B, Edward PN, Mark H, Maret GT, Carroll EC, dan Lester P. Smoking and exposure to environmental tobacco smoke decrease some plasma antioxidants and increase tocopherol in vivo after adjusment for dietary Antioxidants intakes. Am J Clin Nutr. 2003; 77:160-6.
7.Elekofehinti OO, Adanlawo IG, Saliu JA, Sodehinda SA. Saponins from Solanum anguivi fruits exhibit hypolipidemic potential in Rattus novergicus. Der Pharmacia Lettre. 2012: 4 (3) 811-814.
8.Kim JM, Lee EK, Kim DH, Yu BP,Chung HY. Kaempferol modulates pro-inflammatory NF-kappaB activation by suppressing advanced glycation endproduct-induced NADPH oxidase. The Official Journal of the American Aging Association. 2010; 32(2):197–208.
9.Kristanti AN, Aminah NS, Tanjung M, Kurniadi B. Buku Ajar Fitokimia. Surabaya: Airlangga University Press. 2008. Hal 48-49.
10.Lenzatti Manuella, Alan Lopes, Thiago S. Ferreira, Roberto Soares de
Moura, Angela Resende, Luis Cristovao Porto, Samuel Santos Valenc. Mate Tea Ameliorates Emphysema In Cigarette Smoke-Exposed Mice. Experimental Lung Research. 2011 Juni-Oktober. ISSN: 0190-2148. 246-257.
11.Mahmud ZA, Bachar SC, Qais N. Antioxidant and hepatoprotective activities of ethanolic extracts of leaves of Premna esculenta roxb. Against carbon tetrachloride-induced liver damage in rats. J Young Pharmacists. 2012: 4:228-234.
12.Muhammad Ismiyati. Efek Antioksidan Vitamin C terhadap Tikus (Rattus norvegicus) Jantan Akibat Pemaparan Asap Rokok. Tesis. 2009. Sekolah Pascasarjana Institut Pertanian Bogor.
13.NgTB, Cheung Randy CF, Ye X J, Wong Jack H and Ye X Y. Protease Inhibitors, Lectins, Antifungal Protein and Saponins in Soybean. www.intechopen.com. Diunduh tanggal 16 Agustus 2014.
14.Nurliani Anni, Santoso Heri Budi, Rusmiati. Efek Antioksidan Ekstrak Bulbus Bawang Dayak (Eleutherine palmifolia) pada Gambaran Histopatologis Paru-paru Tikus yang Dipapar Asap Rokok. Bioscintiae.
2012.9(1): 60-69.
15.Raaman, N. Phytochemical Technique. New Delhi: New India Publishing Agency; 2006. Hal 21-22, 230.
16.Robinson, T. The Organic Constituents of Higher Plants Their Chemistry and Interrelationships. 5th Ed. North Amherst: Cordus Press; 1983.
17.Samuel Santos, Valenc A, Katia Da Hora, Paulo Castro, Vera Gonc, Alves
Moraes, La ́Is Carvalho, Lu ́Is Crist Ov ̃Ao De Moraes Sobrino Porto. Emphysema and Metalloelastase Expression in Mouse Lung Induced by Cigarette Smoke. Toxicologic Pathology.2004 April. ISSN: 0192-6233. 32(3) : 351-356.
18.Selvam Thamizh. N, Vengatakrishnan V, Damodar Kumar S, Murugesan.S. Evaluation of tissue level antioxidant activity of Premna serratifolia leaf in paracetamol intoxicated wistar albino rats. International Journal of Pharmacy & Life Sciences.2010; 1(2): 86-92.
19.Suhartono E, Fachir H, Setiawan B. Rokok sebagai sumber radikal bebas dalam Kapita selekta biokimia : Stres oksidatif dasar & penyakit. Banjarmasin: Pustaka Banua; 2007.Hal 117-8.
20.Vadivu R, Suresh AJ, Girinath K, Kannan PB, Vimala R dan Kumar NMS. Evaluation of Hepatoprotective and In-vitro Cytotoxic Activity of Leaves of Premna serratifolia Linn. Journal of Scientific Research Publications Department of Pharmacognosy College of Pharmacy Madras Medical College. 2008: 157-164.
21.Voight R. Buku Pelajaran Teknologi Farmasi. Yogyakarta: UGM Press;
1995. Hal 561,567-569,577.
22.Worl Health Organization. WHO Report : The Global Tobacco epidemic [Internet]. 2013 [Dikutip 11 Oktober 2013]. Tersedia dari:
http://www.who.int/tobacco/surveillance/policy/country_profile/idn.pdf
Published
2017-03-24
How to Cite
Tohomi, K. L., Iswahyudi, I., & Wahdaningsih, S. (2017). AKTIVITAS ANTIOKSIDAN EKSTRAK ETANOL DAUN BUAS-BUAS (Premna cordifolia Linn.) TERHADAP GAMBARAN HISTOPATOLOGI PARU TIKUS (Rattus norvegicus) WISTAR JANTAN PASCA PAPARAN ASAP ROKOK. Journal of Tropical Pharmacy and Chemistry, 2(4), 212-224. https://doi.org/10.25026/jtpc.v2i4.67