• Mirhansyah Ardana
Keywords: Testosterone, Electrochemiluminescence, Immunoassay


The research about “The Influence of Sampling Time to Testosterone for Medical Check Up†had been develoved to 19 man aged 19 – 25 years old. Sample was collected at 8 a.m. and 12 a.m. to determine testosterone serum level. Determining of testosteron serum level was done using Electrochemiluminescence Immunoassay(ECLIA). The result show that testosterone serum level mean at 8 a.m. (608.90 ng/dL) is slightly lower than testosterone serum level at 12 a.m. (647.95 ng/dL). The analysis of t test (α=0.05) show that there was not significantly difference between testosteron level mean at 8 a.m. and 12 a.m. So the sampling time to determine testosteron serum level to men can be done until 12.00 a.m.


Keywords: Testosterone, Electrochemiluminescence, Immunoassay



Telah dilakukan penelitian mengenai “Pengaruh Waktu Pengambilan Sampel Darah Terhadap Pemeriksaan Testosteronâ€. Sampel darah diambil dari 19 pria berumur 19 – 25 tahun. Sampel darah dikumpulkan pada pukul 08.00 dan pukul 12.00 untuk pemeriksaan kadar testosteron. Penentuan kadar testosteron dilakukan menggunakan metode Electrochemiluminescence Immunoassay (ECLIA). Hasil penelitian menunjukan bahwa kadar testosteron rata-rata pada pukul 08.00 (608,90 ng/dL) sedikit lebih rendahdaripada kadar testosteron rata-rata pada pukul 12.00 (647,95 ng/dL). Hasil analisis uji t (α = 0,05) menunjukan bahwa tidak terdapat perbedaan yang signifikan antara kadar testosteron rata-rata pada pengambilan pukul 08.00 dan pukul 12.00. Sehingga pengambilan sampel darah untuk pemeriksaan kadar testosteron pada pria dewasa dapat dilakukan hingga pukul 12.00.


Kata kunci: Testosteron, Electrochemiluminescence Immunoassay


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1.Cauter, E. V. & F. W. Turek. 1995. Endocrine and other Biological Rhythms. Endocrinology. Third edition. Edited by Lessie J. DeGroot. Philadelphia: W.B. Saunders Company. Page: 2393-2394
2.Daftary, S. S. & A. C. Core. 2005. IGF-1 in the Brain as a Regulator of Reproductive Neuroendocrine Function. The Society for Experimental and Medicine. 1535-3702.
3.Faldikova, L., I.Diblikova, J. Canderle, Z. Zraly, Z. Vaznik and A. Sulcova.
2001. Effect of Nutrition, Social Factor and Chronic Stress on the Mouse Leydig Cell. Ved. Med.-Czech,46, 2001 (6): 160-168
4.Ganiswarna, S. G. 1995. Farmakologi dan Terapi. Edisi ke-4. Jakatra : Gaya Baru. Hal: 456
5.Hoan Tjay, T. dan Kirana Raharja. 2002. Obat-obat Penting. Jakarta : PT Gramedia. Hal: 641
6.Japardi, I. 2002.Gangguan Tidur.Universitas Sumatera Utara Digital Library.
7.Mitamura, R., K. Yano, N. Suzuki, Y. Ito, Y. Makita and A. Akuno.1999. Diurnal Rhythms of Luteinizing Hormone, Follicle-Stimulating Hormone,
and Testosterone Secretion before the Onset of Male Puberty. The Journal of Clinical Endockrinology and Metabolism. Vol. 84, No. 1 29-37. available at: [diaksesDesember 2006]
8.Pesce, A. J. and L. A. Kaplan. 1987. Mannual Handbook of Clinical Chemistry. Missouri : The C. V. Mosby Company. Page: 266-269
9.Rickenlund, A., M. Thoren, K. Carlstrom, B. V. Schoultz and A. L. Hirschberg.2004.Diurnal Profiles of Testosterone and Pituitary Hormones Suggest Different Mechanisms for Menstrual Disturbances in Endurance Athletes. The Journal of Clinical Endocrinology & Metabolism. Vol. 89, No. 2 702-707
10.Setiyohadi, B. 2000. Osteoporosis Akibat Steroid. Cermin Dunia Kedokteran. no. 129. available at:[diaksesDesember 2007]
11.Tieztz, N. W., C. A. Burtis and E. R. Ashwood. 1994. Tietz Textbook of Clinical Chemistry. Second edition. Edited by Carl A. Burtis and Edward
R. Ashwood. Pennsylvania : W. B. Sauders Company. Page: 1843
12.Williams, R. H.& P. R. Larsen. 2003. Williams Textbook of Endokrinology. Tenth edition. Pennsylvania: Elsevier Science.
13.Winter, S. J., MD. Current Status of Testosterone Replacement Therapy in Men. Arch Fam Med.1999;8:257-263.avaible at: [diaksesDesember 2006]
How to Cite
Ardana, M. (2015). PENGARUH WAKTU PENGAMBILAN SAMPEL DARAH TERHADAP PEMERIKSAAN TESTOSTERON. Journal of Tropical Pharmacy and Chemistry, 3(1), 22-28.