PROFIL KROMATOGRAFI SENYAWA AKTIF ANTIOKSIDAN DAN ANTIBAKTERI FRAKSI N-HEKSANA DAUN LIBO (FICUS VARIEGATA BLUME)

Keywords: n-hexane fraction of Libo leaves (Ficus variegata Blume.), chromatography, antioxidant, antibacterial

Abstract

Research about chromatographic profile of antioxidant and antibacterial compounds in n-hexane fraction of Libo leaves (Ficus variegata Blume.) has been done in order to know the form of chromatographic profile of antioxidant and antibacterial compounds in n-hexane fraction of Libo leaves. Extraction was done by maceration using methanol. Isolation was done by 2 methods, vacuum liquid chromatography (VLC) then followed by conventional column chromatography. Eluent used are n-hexane-ethyl acetate and methanol-chloroform. The results based on conventional column chromatography are as many as 10 fractions. The fractions are being conducted then by a qualitative testing of the antioxidant activity using DPPH method and antibacterial activity test using bioautography TLC method. The results formed as a chromatographic profile of fraction that contain antioxidant and antibacterial activity, which are as many as 5 fractions (NHB1, NHB3, NHB4, NHC1, NHC3) active as an antioxidant and 4 fractions (NHB1, NHC1, NHC2, NHC3) which are active as an antibacterial. The secondary metabolite contents of the fractions are alkaloid, flavonoid and steroid/terpenoid.

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Published
2015-12-31
How to Cite
Rusli, R., Hardina, M. P., Muflihah, F., & Rahmadani, A. (2015). PROFIL KROMATOGRAFI SENYAWA AKTIF ANTIOKSIDAN DAN ANTIBAKTERI FRAKSI N-HEKSANA DAUN LIBO (FICUS VARIEGATA BLUME). Journal of Tropical Pharmacy and Chemistry, 3(2), 124-130. https://doi.org/10.25026/jtpc.v3i2.98

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