FORMULASI NANOPARTIKEL EKSTRAK BAWANG DAYAK (Eleutherine americana (Aubl) Merr) DENGAN VARIASI KONSENTRASI KITOSAN-TRIPOLIFOSFAT (TPP)

  • Ermina Pakki
  • Sumarheni Sumarheni
  • Aisyah F
  • Ismail Ismail
  • Syarfina Safirahidzni
Keywords: Bawang dayak ( Eleutherine americana (Aubl) Merr.), antioksidan, nanopartikel, kitosan, tripolifosfat, gelasi ionik

Abstract

Ekstrak etanol Bawang dayak (Eleutherine americana (Aubl) Merr.) diketahui memiliki aktivitas antioksidan yang kuat sehingga berpotensi untuk dikembangkan menjadi sistem penghantaran nanopartikel. Penelitian ini bertujuan untuk mengetahui pengaruh variasi konsentrasi kitosan – tripolifosfat (TPP) terhadap karakteristik fisik dari nanopartikel. Ekstrak bawang dayak diformulasi dalam bentuk nanopartikel dengan metode gelasi ionik dengan variasi konsentrasi polimer kitosan : tripolifosfat yaitu 0,5% : 0,5% (F1), 0,75% : 0,5% (F2), dan 1% : 0,5% (F3). Parameter pengujian meliputi penentuan ukuran dan indeks polidispersitas nanopartikel menggunakan particle size analyzer, pengamatan morfologi menggunakan scanning electron microscopy, pengukuran efisiensi penjerapan, dan disolusi in vitro. Hasil penelitian menunjukkan bahwa nanopartikel F1, F2, dan F3 memiliki ukuran masing-masing sebesar 256,30 nm, 376,28 nm dan 419,18 nm dengan distribusi ukuran yang relatif homogen dan efisiensi penjerapan masing-masing sebesar 69,54%, 77,51% dan 79,79%. Pengamatan morfologi dari nanopartikel menunjukkan bentuk partikel yang mendekati spheris (bulat) dengan permukaan yang kasar. Profil pelepasan obat dari nanopartikel F1, F2, dan F3 pada jam ke-8 masing-masing sebesar 71,19 % (F1), 74,97% (F2) dan 80,55% (F3). Berdasarkan hasil penelitian yang telah dilakukan, dapat disimpulkan bahwa ekstrak bawang dayak dapat diformulasi dalam ukuran nanopartikel dengan karakteristik fisik yang bervariasi tergantung pada konsentrasi kitosan dan tripolifosfat yang digunakan.

Kata Kunci:   Bawang dayak (Eleutherine americana (Aubl) Merr.), antioksidan, nanopartikel, kitosan, tripolifosfat, gelasi ionik.

ABSTRACT

Bawang dayak (Eleutherine americana (Aubl) Merr.) ethanol extract had been known to have excellent antioxidant activity that has the potential to be developed into a nanoparticle delivery systems. This study aims to determine the effect of varying concentrations of chitosan - tripolyphosphate (TPP) to the physical characteristics of nanoparticles. Bawang dayak extract formulated in the form of nanoparticles with an ionic gelation method and and using polymers chitosan - tripolyphospate varying concentration as 0.5% : 0.5% (F1), 0.75% : 0.5% (F2), and 1% : 0.5% (F3). The measured parameters were determinating particle size and polydispersity index using particle size analyzer, observation of particle morph using scanning electron microscopy, measurement of entrapment efficiency and dissolution in vitro. The result showed that the average size of nanoparticle F1, F2, and F3 respectively is 256.30 nm, 376 nm and 419.18 nm with entrapment efficiency respectively is 69.54% (F1), 77.51% (F2) and 79.79% (F3) with a relatively homogenous size distribution and entrapment efficiencies respectively is 69.54%, 77.51% and 79.79%. Observation of morphology of the nanoparticles shows the particle shape is almost sphere (spherical) with a rough surface. The profile of drug release from the nanoparticles F1, F2, and F3 in 8 hours respectively is 71.19% (F1), 74.97% (F2) and 80.55% (F3). Based on this study, it was concluded that the etanol extract of bawang dayak can be formulated into nanoparticles with different physical characteristics based on the ratio of polymer chitosan and tripolyphosphate used.

Keywords:     Bawang dayak (Eleutherine americana (Aubl) Merr.), nanoparticles, chitosan, tripolyphosphate, ionic gelation.

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References

1.Febrinda AE, Astawan M, Wresdiyati T dan Yuliana ND. Kapasistas antioksidan dan inhibitor alfa glucosidase ekstrak umbi bawang dayak.
Jurnal teknologi dan Industri Pangan. 2013. 24 (2); pp. 161-167.
2.Kuntorini EM, Astuti MD. Penentuan Aktivitas Antioksidan EkstrakEtanol Bulbus Bawang Dayak (Eleutherine americana Merr).
Sains dan Terapan Kimia. 2010. 4 (1); pp. 15 –22.
3.Ifesan B, Ibrahim D dan Voravuthikunchai SP. Antimicrobial Activity of Crude Ethanolic Extract From Eleutherine americana. Journal of Food, Agriculture & Environment. 2010. 8 (3 & 4); pp. 1233-1236.
4.Fitri Y, Rosidah dan Suwarso E. Effects of inhibition cell cycle an apoptosis of sabrang onion extract (Eleuthetine bulbosa(Mill.) Urb.) on breast cancer cells. International Journal of PharmTech Research. 2014. 6 (4); pp. 1392-1396.
5.Pratiwi D, Wahdaningsih S, Isnindar. The Test of Antioxidant Activity from Bawang Mekah Leaves (Eleutherine americana Merr.) using DPPH (2,2-Diphenyl-1-Picrylhydrazyl) Method. Traditional Medicine Journal. 2013. 18 (1); pp. 9-16.
6.Ridwan I. Pengaruh Konsentrasi Cairan Penyari Etanol Terhadap Kadar Fenolik Total Dan Aktivitas Antioksidan Ekstrak Umbi Bawang
Dayak (Eleutherine americana (Aubl) Merr). Skripsi
Program Studi Farmasi Fakultas Farmasi Universitas Hasanuddin. Makassar. 2015.
7.Martien R, Adhyatmika, Iramie DK, Farida V, Purwita SD. Perkembangan Teknologi Nanopartikel Sebagai Sistem Penghantaran Obat. Majalah Farmaseutik. 2012. 8(1); pp. 133-144.
8.Dewandari KT, Yuliani S, Yasni S. Ekstraksi dan Karakterisasi
Nanopartikel Ekstrak Sirih Merah(Piper crocatum). Jurnal Pascapanen. 2013. 10(2); pp. 58-65.
9.Kaban J, Bangun HD, Asteria KD. Pembuatan Membran Kompleks
Polielektrolit Alginat Kitosan. Jurnal Sains Kimia. 2006. 10 (1); pp. 10-
16.
10.Shu XZ, Zhu KJ. Controlled Drug Release properties of ionically Cross-linked chitosan beads : The influence of anion structure.
International Journal of Pharmaceutics. 2002. 233 (1-2); pp.217
-255.11.Liu H, Gao C. Preparation and properties of ionically cross-
linked chitosan nanoparticles. Polymers for Advance Technologies.
2009. 20 (7); pp. 613–619.
12.Wang N, Zhang M. Chitosan-Based Hydrogels for Controlled, Localized Drug Delivery. Advanced Drug Delivery Review. 2010. 9 (1); pp. 83–99.
13.Anggasari N, Alauhdin M, Tri AP, Sintesis dan Karakterisasi Membran Kitosan-Tripolifosfat sebagai Alternatif Pengontrol Sistem Pelepasan Obat. Indonesian Journal of Chemical Science. 2013. 2 (3); pp. 190-193.
14.Stoica R, Şomoghi R, Ion RM. Preparation of Chitosan-Tripolyphosphate Nanoparticles For The Encapsulation of Polyphenols Extracted FromRose Hips. Digest Journal of Nanomaterials and Biostructures. 2013. 8 (3); pp. 955 –963.
15.Zhang H, Allen, C, Kumacheva, E. Monodisperse chitosan nanoparticles for mucosal drug delivery. Biomacromolecules. 2004. 5
; p. 2461.
16.Calvo P, Remun ̃an-Lopez C, Vila-Jato JL, Alonso MJ. Chitosan and chitosan/ethylene oxide-propylene oxide block copolymer nanoparticles as novel carriers for proteins and vaccines.Pharm Res
. 1997.14; pp.1431-1436.
17.Swarbrick J. Encyclopediaof pharmaceutical technology. 3rd ed.
Vol. 4. Informa Healthcare USA Inc : New York. 2007.
18.Tiyaboonchai, W. Chitosan Nanoparticles:A Promising System for
Drug Delivery. Naresuan University Journal. 2003. 11(3); pp. 51-66.
19.Liu H, Changyou G. Preparation and properties of ionically cross-linked chitosan nanoparticles. Polym. Adv. Technol. 2009. 20; pp. 613
–619.
20.Dunne M, Corrigan OI, Ramtoola Z. Influence of particle size and dissolution conditions on the degradation properties of polylactide-co
-glycolide particles. Biomaterials. 2000. 21; pp. 1659-1668.
Published
2016-12-31
How to Cite
Pakki, E., Sumarheni, S., F, A., Ismail, I., & Safirahidzni, S. (2016). FORMULASI NANOPARTIKEL EKSTRAK BAWANG DAYAK (Eleutherine americana (Aubl) Merr) DENGAN VARIASI KONSENTRASI KITOSAN-TRIPOLIFOSFAT (TPP). Journal of Tropical Pharmacy and Chemistry, 3(4), 251-263. https://doi.org/10.25026/jtpc.v3i4.113