Riset Sitotoksik Campuran Ekstrak Daun Sirsak (Annona muricata L) dan Kulit Buah Manggis (Garcinia mangostana L) pada Sel Vero dan AML12

  • Tuty Erlina Mardja Pusat Riset Obat dan Makanan, Badan POM RI, Jakarta, Indonesia
  • Fitria Rahmi Pusat Riset Obat dan Makanan, Badan POM RI, Jakarta, Indonesia
  • Eka Rusmawati Pusat Riset Obat dan Makanan, Badan POM RI, Jakarta, Indonesia
  • Rina Adriany Pusat Riset Obat dan Makanan, Badan POM RI, Jakarta, Indonesia
  • Murtiningsih Murtiningsih Pusat Riset Obat dan Makanan, Badan POM RI, Jakarta, Indonesia
  • Herlina B. Setijanti Pusat Riset Obat dan Makanan, Badan POM RI, Jakarta, Indonesia
  • Tepy Usia Pusat Riset Obat dan Makanan, Badan POM RI, Jakarta, Indonesia
Keywords: Sitotoksik, Daun sirsak, Kulit buah manggis, sel vero, sel AML12, IC 50

Abstract

Campuran Daun sirsak dan Kulit buah manggis sekarang ini banyak digunakan sebagai fito-terapi penyakit kanker dan antioksidan. Tetapi penggunaan bahan ini belum diketahui data keamanannya. Tujuan dilakukan penelitian adalah untuk mengetahui efek sitotoksik campuran ekstrak daun sirsak dan kulit buah manggis terhadap sel vero dan sel AML12. Uji sitotoksik dapat memprediksi keberadaan senyawa yang bersifat toksik secara in vitro menggunakan sel normal atau sel yang telah mengalami transformasi. Uji ini menggunakan 2 jenis cell line, yaitu sel vero dan sel AML12. Sampel berupa simplisia daun sirsak dan kulit buah manggis yang dibuat ekstrak dengan menggunakan etanol 96%. Konsentrasi uji yang digunakan adalah 100; 50; 25; 12,5; 6,25 dan 3,125 mg/mL untuk sel vero dan 500; 250; 125; 62,5; 31,25 dan 15,625 mg/mL untuk sel AML12. Kultur sel dilakukan di wellplate 96 diinkubasi didalam inkubator CO2 pada suhu 37°C selama 24 jam kemudian ditambahkan sampel uji dan selanjutnya diinkubasi kembali didalam inkubator CO2 pada suhu 37°C selama 24 jam. Langkah selanjutnya uji MTT dan kemudian dibaca dengan ELISA reader pada panjang gelombang 570nm. Diperoleh hasil sebagai berikut: IC50 55,97 mg/mL pada sel vero, dan 43,292 mg/mL pada sel AML12. Kesimpulannya sampel campuran ekstrak daun sirsak dan kulit buah manggis ini mempunyai efek toksik terhadap sel vero dan sel AML12 (IC50<100 mg/mL).

Kata kunci: Sitotoksik, Daun sirsak, Kulit buah manggis, sel vero, sel AML12, IC50

Abstract

Mix of soursop leaves and mangosteen pericarp use as cancer phytotherapy and antioxidant, but it is not yet known its toxicities data. The aim of this study was evaluate cytotoxicity effect of mix of soursop leaves and mangosteen pericarps extract on vero cells and AML12 cells. Toxicity study is one ways to predict the presence of toxic compounds using normal cells or cells that have undergone a transformation. That study was using vero cell and AML12. Samples was ethanolic extract of soursop leaves and mangosteen pericarp. The test dose were 100; 50; 25; 12,5; 6,25 dan 3,125 mg/mL in vero cell and 500; 250; 125; 62,5; 31,25 dan 15,625 mg/mL in AML12. Cells were cultured in well plate 96 and incubated in CO2, temperature 37°C for 24 hours and then added samples and incubated incubated in CO2, temperature 37°C for 24 hours. Cell was added MTT and read with ELISA reader. The results showed IC50 55,97mg/mL in vero cells and 43,292 mg/mL in AML12. Conclusion of this study is ethanolic extract of soursop leaves and mangosteen pericarps has toxicity effect in vero cells and AML12 cells (IC50<100 mg/mL).

Keywords: cytotoxiciy, soursop leaves, mangosteen pericarp, vero cells, AML12 cells, IC50

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References

1.Olakunle, Sanni, Obidoa Onyechi, Omale James, 2014, Toxicity,anti -lipid peroxidation, invitro and invivo evaluation of antioxidant activity of Annona muricata ethanol stem bark extract, American Journal of Life Sciences 2014; 2(5): 271-277
2.Solomon-Wisdom, G.O, Ugoh, S.C
and Mohammed, .B., 2014, Phytochemical Screening and Antimicrobial activities of Annona muricata (L) leaf extract, American
Journal of Biological, Chemical and Pharmaceutical Sciences Vol. 2, No. 1, January 2014, PP: 01-07, ISSN: 2328 -6814 (Online), Available online at http://www.ajbcps.com/
3.Pieme, Constant Anatole, Santosh Guru Kumar, Mireille Sylviane Dongmo, Bruno Moukette Moukette, Fabrice Fekam Boyoum, Jeanne Yonkeu Ngogang and Ajit Kumar Saxena, Antiproliferative activity and
induction of apoptosis by Annona muricata (Annonaceae) extract on human cancer cells, BMC Complementary and Alternative Medicine 2014, 14:516, http://www.biomedcentral.com/1472-6882/14/516
4.Moongkarndi, Primchanien, Nuttavut Kosema, Sineenart Kaslungka, Omboon Luanratana, Narongchai Pongpan, Neelobol Neungton,2004, Antiproliferation, antioxidation and induction of apoptosis by Garcinia
mangostana (mangosteen) on SKBR3 human breast cancer cell line, Journal of Ethnopharmacology 90 (2004) 161–166, http://livingbyheart.tripod.com/sitebuildercontent/sitebuilderfiles/-garcinia_mangostana_breast_cancer_study.pdf
5.Obidike Ifeoma and Salawu Oluwakanyinsola, 2013, Screening of Herbal Medicines for Potential Toxicities, New Insights into Toxicity and Drug Testing, intech, http://cdn.intechopen.com/pdfs wm/42020.pdf
6.Riss,Terry L,Richard A Moravec,Andrew L Niles,Helene A Benink,Tracy J Worzella,and Lisa Minor, 2013, Cell Viability Assays,
Assay Guidance Manual [Internet], PublishedMay 1, 2013, http://www.ncbi.nlm.nih.gov/books/NBK144065/, tanggal 21 April 2015
7.Quispe, Angel, David Zavala, Margarita Posso, José Rojas, Abraham Vaisberg, 2007, Annona muricata(Guanabana) Citotoxic Effect In Lung And Abdominal Neoplasms Cell Lines, CIMEL 2007 Vol. 12 Nº 1
8.Padmaa PM, Chansouria JPN, Khosa RL.Wound Healing Activity of Annonamuricata extract. J Pharm Res.2009;2(3):404-6.
9.Yang,H. J., X. Li, N. Zhang, J. W. Chen and M. Y. Wang, “Two New Cytotoxic Acetogenins from Annona squamosa,” Journal of Asian Natural Products Research, Vol. 11, No. 3, 2009, pp. 250-256. doi:10.1080/10286020802682916
10.Morré DJ, de Cabo R, Farley C, OberliesNH, McLaughlin JL. Mode of action ofbullatacin, apotent antitumor acetogenin:Inhibition of NADH oxidase activity of HeLaand HL-60, but not liver, plasmamembranes. Life Sci. 1995;56:343-8.
11.Kojima N, Tanaka T. Medicinal Chemistryof
Annonaceous Acetogenins: Design,Synthesis, and Biological Evaluation ofNovel Analogues. Molecules.2009;14:3621-61.
12.Anne-Isabelle, W., R. Hocquemiller, A. Laurens and A. Cavé, “Glaucafilin, an Acetogenin from Annonaglauca,” Journal of Phytochemistry, Vol. 44, No. 8, 1997, pp. 1537-1540
13.Rivanti,Erlina, Annishfia Lailatur Rohmah, Herwandhani Putri, Prisnu Tirtanirmala, and Dyaningtyas Dewi PutriPamungkas, 2012, Ethanolic Extract ofMangosteen (Garcinia mangostana) Peel Inhibits T47Dand Hela CellsLine Proliferation Via Nf-Ò›BPathway Inhibition, Indonesian Journal of Cancer Chemoprevention, 2012, 3(2): 392-398ISSN: 2088 -0197(13 Januari 2016)
14.Aisha, AbdalrahimFA, KhalidMAbu-Salah, ZhariIsmail andAmin MalikShahAbdulMajid, 2012, In vitro and in vivo anti-colon cancer
effects of Garcinia mangostanaxanthones extract, BMC Complementary and Alternative Medicine
15.Sato A, Fujiwara H, Oku H, Ishiguro K, Ohizumi Y. 2004. Alphamangostininduces Ca2+-ATPase-dependent apoptosis via mitochondrial pathway in PC12 cells. J Pharmacol Sci 95:33–40
Published
2016-12-31
How to Cite
Mardja, T. E., Rahmi, F., Rusmawati, E., Adriany, R., Murtiningsih, M., Setijanti, H. B., & Usia, T. (2016). Riset Sitotoksik Campuran Ekstrak Daun Sirsak (Annona muricata L) dan Kulit Buah Manggis (Garcinia mangostana L) pada Sel Vero dan AML12. Journal of Tropical Pharmacy and Chemistry, 3(4), 283-290. https://doi.org/10.25026/jtpc.v3i4.116