Antidiabetic Activities of Broccoli Extracts (Brassica oleracea L.var italica) on Mice Induced Streptozotocin-Nicotinamide

  • Heni Lutfiyati Universitas Muhammadiyah Magelang, Indonesia
  • Fitriana Yuliastuti Pharmacy Technician Diploma Program, Faculty of Health Sciences, Universitas Muhammadiyah Magelang, Indonesia
  • Imron Wahyu Hidayat Department of Pharmacy, Faculty of Health Sciences, Universitas Muhammadiyah Magelang, Indonesia
  • Prasojo Pribadi Department of Pharmacy, Faculty of Health Sciences, Universitas Muhammadiyah Magelang, Indonesia
  • Sodiq Kamal Department of Nursing, Faculty of Health Sciences, Universitas Muhammadiyah Magelang, Indonesia
Keywords: antidiabetic activities, broccoli extract, streptozotocin-nicotinamide


Several plant species have been used for the treatment of diabetes. Research on plants that can be used to treat diabetes such as Cinnamomum cassia and Cinnamomum zeylanicum, Piper sarmentosum Roxb, Nelumbo nucifera, Ceiba petandra Memecylon malabaricum. Broccoli (Brassica oleracea L var italics) is a flower of vegetables like cabbage plants. Broccoli juice is made capable of direct and indirect effects on the reduction of blood LDL; the indirect effect is by repairing pancreatic beta cells and increasing insulin sensitivity. The purpose of this study was to determine whether broccoli has activity as antidiabetic. The subjects consisted of 15 mice induced with nicotinamide 70 mg/kg BW and Stz 150 mg/kg BW. Diabetic mice were divided into the positive control group (glibenclamide), negative control group (CMC Na) and three treatment groups. The test material is broccoli extract dose 75 mg / kg BW, 150 mg /kg BW and 300 mg/kg BW given peroral for 14 days. Anova test results showed that there was a significant difference in the average percentage of decreased blood glucose levels in all treatments. The result of Post Hock Tukey HSD test with a 95% confidence level showed a significant difference in the negative control group compared with positive control group and extract 300mg / kg BW. This shows that positive control group and extracts 300mg / kg body weight have an activity to decrease blood glucose level.


Download data is not yet available.


S. Pramono, F. Farmasi, and U. Gadjah, “KONTRIBUSI BAHAN OBAT ALAM,” J. Bahan Alam Indones., 2002.

L. Oktora, R. Kumala, S. Pengajar, P. Studi, and F. Universitas, “PEMANFAATAN OBAT TRADISIONAL DAN KEAMANANNYA,” vol. III, no. 1, pp. 1–7, 2006.

E. N. Eugen J. Vershpol, Katrin Bauer, “Antidiabetic effect of Cinnamomum cassia and Cinnamomum zeylanicum In vivo and In vitro,” Phyther. Res., vol. 19, no. 3, pp. 203–206, 2005.

S. K. Penchom Peungvichaa, Suwan S. Thirawaparana, Rungravi Temsiririkkulb, Hiroshi Watanabec, Jeevan Kumar Prasaind, “Hypoglycemic effect of the water extract of Piper sarmentosum in rats.” pp. 27–32, 1998.

B. P. S. Pulok K. Mukherjee, Kakali Saha, M.Pal, “Effect of Nelumbo nucifera rhizome extract on blood sugar level in rats.” pp. 207–213, 1997.

O. O. Elekofehinti, “Saponins : Anti-diabetic principles from medicinal plants – A review,” Pathophysiology, vol. 22, no. 2, pp. 95–103, 2015.

S. I. T Amalraj, “Evaluation of the hypoglycaemic effect of Memecylon umbellatum in normal and alloxan diabetic mice,” J. Ethnopharmacol., vol. 62, no. 3, pp. 247–250, 1998.

Setyadi, W. Yulian, L. Yuliatun, and Lowita, “Jus Brokoli Menurunkan Kadar Low Density Lipoprotein Darah pada Tikus Model Diabetes Melitus Broccoli Juice Reduce Blood Low Density Lipoprotein in Diabetes Mellitus Mice Model,” J. Kedokt. Brawijaya, vol. 28, no. 1, pp. 26–29, 2014.

Z. Bahadoran, M. Tohidi, P. Nazeri, M. Mehran, F. Azizi, and P. Mirmiran, “Effect of broccoli sprouts on insulin resistance in type 2 diabetic patients: a randomized double-blind clinical trial,” Int. J. Food Sci. Nutr., vol. 63, no. 7, pp. 767–771, 2012.

N. E. Wulandari, P. Studi, I. Gizi, F. Kedokteran, and U. Diponegoro, “TERHADAP KADAR GLUKOSA DARAH PUASA Effect of Steamed Broccoli ( Brassica Oleracea ) on Fasting Blood Glucose Level in Pre-diabetes Women,” 2014.

K. Hideaki et al., “Be n e fi cial Effects of Antioxidants in Diabetes Possible Protection of Pancreatic !-Cells Against Glucose Tox i c i t y .pdf,” Diabetes, vol. 48, 1999.

Lloyd R, Synder, J. J. Kirland, and J. L.Glajch, “Practical HPLC Method Development 2nd Edition.” John Wiley, New York, 1997.

M. Sangi, M. R. J. Runtuwene, and H. E. I. Simbala, “DI KABUPATEN MINAHASA UTARA,” vol. 1, no. 1, pp. 47–53, 2008.

T. Aniszewski, Alkaloid-Secrets of Life. Elsevier, Oxford, 2007.

Z. Bahadoran, P. Mirmiran, F. Hosseinpanah, M. Hedayati, S. Hosseinpour-Niazi, and F. Azizi, “Broccoli sprouts reduce oxidative stress in type 2 diabetes: a randomized double-blind clinical trial.,” Eur. J. Clin. Nutr., vol. 65, no. 8, pp. 972–7, 2011.

A. Rao and Gurfinkel, “The Bioactivity of Saponins : Triterpenoid and Steroidal Gycosides,” 2011.

M. Kumari and S. Jain, “Tannins : An Antinutrient with Positive Effect to Manage Diabetes,” vol. 1, no. 12, pp. 70–73, 2012.

M. A. Shah, M. M. R. Sarker, and M. Gousuddin, “Antidiabetic potential of Brassica Oleracea var. Italica in type 2 diabetic sprague dawley (sd) rats,” Int. J. Pharmacogn. Phytochem. Res., vol. 8, no. 3, pp. 462–469, 2016.

V. Patel and V. Sharma, “Effect of Brassica oleracea Extracts on Blood Glucose and Antioxidant Profile in Streptozotocin Induced Diabetic Rats,” vol. 1, no. 5, pp. 5–10, 2014.

R. Hendra, S. Ahmad, A. Sukari, and M. Y. Shukor, “Flavonoid Analyses and Antimicrobial Activity of Various Parts of Phaleria macrocarpa ( Scheff .) Boerl Fruit,” pp. 3422–3431, 2011.

D. Grassi, G. Desideri, and C. Ferri, “Flavonoids: Antioxidants Against Atherosclerosis,” pp. 889–902, 2010.

R. F. Epand, P. B. Savage, and R. M. Epand, “Bacterial lipid composition and the antimicrobial efficacy of cationic steroid compounds ( Ceragenins ),” vol. 1768, pp. 2500–2509, 2007.


M. O. Fatharanni and D. I. Anggraini, “Efektivitas Brokoli ( Brassica Oleracea var . Italica ) dalam Menurunkan Kadar Kolesterol Total pada Penderita Obesitas,” Majority, vol. 6, no. 1, pp. 64–70, 2017.

How to Cite
Lutfiyati, H., Yuliastuti, F., Hidayat, I. W., Pribadi, P., & Kamal, S. (2018). Antidiabetic Activities of Broccoli Extracts (Brassica oleracea L.var italica) on Mice Induced Streptozotocin-Nicotinamide. Journal of Tropical Pharmacy and Chemistry, 4(4), 182-188.