Effect of Media Types on the Growth of Callus Culture in Kumis Kucing Orthosiphon aristatus (Blume) Miq.

  • Wahyu Widayat Laboratorium Penelitian dan Pengembangan Kefarmasian "Farmaka Tropis", Fakultas Farmasi, Universitas Mulawarman, Samarinda, Indonesia
  • Muhammad Satyo Pradana
  • Mirhansyah Ardana
Keywords: Orthosiphon aristatus, callus Culture, Kumis Kucing, MS Media, WPM media

Abstract

The lack of conventional availability of plant Kumis Kucing makes tissue culture techniques used as a solution to overcome this problem. In tissue culture, media is a major factor in producing a good crop of plantlets. Media Murashige & Skoog (MS), Lloyd & McCown Woody Plant (WPM) media was used in the induction of Orthosiphon aristatus (Blume) Miq cat whiskers callus culture, in this study also used growth regulators in the form of 2,4-D added to each-individual media. The results showed the best callus growth occurred in Murashige & Skoog (MS) media compared to the Lloyd & McCown Woody Plant (WPM) media, where the callus produced was 3.28 g on MS media.

Downloads

Download data is not yet available.

References

[1]Akowuah, G., Zhari, I., Norhayati, I., Sadikun, A., Khamsah, S. 2004. Sinensetin, eupatorin, 3′-hydroxy-5, 6, 7, 4′-tetramethoxyflavone and rosmarinic acid contents and antioxidative effect of Orthosiphon stamineus from Malaysia. Food Chemistry. 87.559-566

[2] Ameer, O. Z., Salman, I. M., Asmawi, M. Z., Ibraheem, Z. O., Yam, M. F. 2012. Orthosiphon stamineus: traditional uses, phytochemistry, pharmacology, and toxicology. Journal of medicinal food. 15.678-690

[3] Aminudin, I. 2005. Bahan Bioaktif Kumis Kucing (Orthosiphon aristatus (Blume) Miq). di Bawah Tegakan Hutan. Makalah Sains Ilmu Kehutanan IPB.

[4] Basheer, M. K. A., Majid, A. M. S. A. 2010. Medicinal potentials of Orthosiphon stamineus Benth. Webmed Central. 1(12).2046-1690

[5] Bhatia, S., Sharma, K., Dahiya, R., Bera, T. 2015. Modern applications of plant biotechnology in pharmaceutical sciences. Academic Press Elsevier.United Kingdom

[6] Bhojwani, S. S., Razdan, M. K. 1986. Plant tissue culture: theory and practice. Elsevier.Netherland U.S.A

[7] Coto, I. Z., Hardjanto, I. 2005. Bahan bioaktif kumis kucing (Orthosiphon aristatus) di bawah tegakan hutan. Falsafah Sains Institut Pertanian Bogor. 702.8

[8] Daslina, D., Darwin, E., Djamal, A. 2015. Pengaruh Pemberian Glutamin pada Kemampuan Fagositosis Makrofag terhadap Pseudomonas aeruginosa. Jurnal Kesehatan Andalas. 4

[9] Dorothy, P., Sudarshana, M., Nissar, A., Girish, H. 2016. In vitro cytological studies of leaf callus cultures of Orthosiphon aristatus (Blume) Miq. British Biotechnology Journal. 13.1-6

[10] Elangomathavan, R., Kalaivanan, P., Hariharan, P., Beaulah, S. 2017. High efficient protocol for Callus induction and Regeneration of a medicinal plant Orthosiphon stamineus. Int. J. of Adv. Research in Biological Sciences. 4.113-122

[11] Fatmawati, A. 2008. Kajian konsentrasi BAP dan 2, 4-D terhadap induksi kalus tanaman Artemisia annua L. Secara in vitro. Fakultas Pertanian UNS. Surakarta

[12] George, E. F., Sherrington, P. D. 1984. Plant propagation by tissue culture. Exegetics Ltd.British.

[13] Hutami, S. 2016. Ulasan masalah pencoklatan pada kultur jaringan. Jurnal Agro Biogen. 4(2).83-88

[14] Ignacimuthu, S., Elangomathavan, R., Prakash, S., Kathiravan, K., Seshadri, S. 2003. High frequency in vitro propagation of kidney tea plant. Plant Cell, Tissue and Organ Culture. 72.83-86

[15] Indah, P. N., Ermavitalini, D. 2013. Induksi Kalus Daun Nyamplung (Calophyllum inophyllum Linn.) pada Beberapa Kombinasi Konsentrasi 6-Benzylaminopurine (BAP) dan 2, 4-Dichlorophenoxyacetic Acid (2, 4-D). Jurnal Sains dan Seni ITS. 2.E1-E6

[16] Lim, F. L., Yam, M. F., Asmawi, M. Z., Chan, L.-K. 2013. Elicitation of Orthosiphon stamineus cell suspension culture for enhancement of phenolic compounds biosynthesis and antioxidant activity. Industrial crops and products. 50.436-442

[17] Mariam, A., Asmawi, M., Sadikun, A. 1996. Hypoglycaemic activity of the aqueous extract of Orthosiphon stamineus. Fitoterapia. 67.465-468

[18] Matsubara, T., Bohgaki, T., Watarai, M., Suzuki, H., Ohashi, K., Shibuya, H. 1999. Antihypertensive actions of methylripariochromene A from Orthosiphon aristatus, an Indonesian traditional medicinal plant. Biological and Pharmaceutical Bulletin. 22.1083-1088

[19] Muhammad Satyo Pradana, Wahyu Widayat, Ardana, M. 2018. Pengembangan Metode Sterilisasi pada Eksplan Guna Meningkatkan Keberhasilan Kultur Tanaman Kumis Kucing (Orthosiphon aristatus (Blume) Miq). Journal of Tropical Pharmacy and Chemistry. 8.

[20] Nezhadahmadi, A., Mohsin, R., Efzueni, S., Azhar, S. 2012. Propagation of Medicinal Plant Orthosiphun Stamineus (Misai Kucing) Through Axillary Branching and Callus Culture. Life Science Journal Acta Zhengzhou University Overseas Edition. 9.5283-5294

[21] Olah, N.-K., Radu, L., Mogoşan, C., Hanganu, D., Gocan, S. 2003. Phytochemical and pharmacological studies on Orthosiphon stamineus Benth.(Lamiaceae) hydroalcoholic extracts. Journal of pharmaceutical and biomedical analysis. 33.117-123

[22] Pierik, R. L. M. 1991. In Vitro Culture of Hinger Plant Micropropagation. Springer.Netherlands.

[23] Reshi, N. A., Sudarshana, M., Rajashekar, N. 2013. Callus Induction and Plantlet Regeneration in Orthosiphon aristatus (Blume) Miq. A Potent Medicinal Herb. IOSR Journal of Pharmacy and Biological Sciences. 6.52-55

[24] Sheena, E., Jeya Jothi, G. 2015. In vitro propagation of Orthosiphon stamineus Benth (Lamiaceae) an important medicinal plant using nodal and leaf explants. The Pharma Innovation Journal. 4.6-10

[25] Sudrajad, H., Suharto, D., Wijaya, N. R. 2016. Inisiasi Kalus Sanrego (Lunasia amara Blanco.) dalam Kultur Jaringan. Proceeding Biology Education Conference: Biology, Science, Enviromental, and Learning. 13.619-623

[26] Sumaryono, W., Proksch, P., Wray, V., Witte, L., Hartmann, T. 1991. Qualitative and quantitative analysis of the phenolic constituents from Orthosiphon aristatus. Planta medica. 57.176-180

[27] Takeda, Y., Matsumoto, T., Terao, H., Shingu, T., Futatsuishi, Y., Nohara, T., dkk. 1993. Orthosiphol D and E, minor diterpenes from Orthosiphon stamineus. Phytochemistry. 33.411-415

[28] Tetsumura, T., Matsumoto, Y., Sato, M., Honsho, C., Yamashita, K., Komatsu, H., dkk. 2008. Evaluation of basal media for micropropagation of four highbush blueberry cultivars. Scientia Horticulturae. 119.72-74

[29] Tezuka, Y., Stampoulis, P., Banskota, A. H., Awale, S., Tran, K. Q., Saiki, I., dkk. 2000. Constituents of the Vietnamese medicinal plant Orthosiphon stamineus. Chemical and pharmaceutical bulletin. 48.1711-1719

[30] Vengadesan, G., Ganapathi, A., Amutha, S., Selvaraj, N. 2002. In vitro propagation of Acacia species a review. Plant Science. 163.663-671

[31] Wai-Leng, L., Lai-Keng, C. 2004. Establishment of Orthosiphon stamineus cell suspension culture for cell growth. Plant Cell, Tissue and Organ Culture. 78.101-106

[32] Yam, M. F., Asmawi, M. Z., Basir, R. 2008. An investigation of the anti-inflammatory and analgesic effects of Orthosiphon stamineus leaf extract. Journal of medicinal food. 11.362-368

[33] Yam, M., Ang, L., Basir, R., Salman, I., Ameer, O., Asmawi, M. 2009. Evaluation of the anti-pyretic potential of Orthosiphon stamineus Benth standardized extract. Inflammopharmacology. 17.50-54
Published
2020-06-30
How to Cite
Widayat, W., Pradana, M. S., & Ardana, M. (2020). Effect of Media Types on the Growth of Callus Culture in Kumis Kucing Orthosiphon aristatus (Blume) Miq. Journal of Tropical Pharmacy and Chemistry, 5(1), 21-28. https://doi.org/10.25026/jtpc.v5i1.210