Molluscicidal activities of Tetrapleura tetraptera fruit extract, Aridanin and their particulate formulations on adult and newly hatched Biomphalaria glabrata snails

  • Ganiyu Akinniyi Drug Research and Production Unit, Faculty of Pharmacy, Obafemi Awolowo University, Ile-Ife, Osun State, Nigeria
  • Funmilola Fisusi Drug Research and Production Unit, Faculty of Pharmacy, Obafemi Awolowo University, Ile Ife, Osun State, Nigeria
  • Bamigboye Taiwo Department of Pharmaceutical Chemistry, Faculty of Pharmacy Obafemi Awolowo University, Ile Ife, Osun State, Nigeria

Abstract

The study investigated the molluscicidal activities of chloroform/methanol (1:1) extract of Tetrapleura tetraptera and isolated Aridanin. It also developed useable particulate formulation of chloroform methanol extract and Aridanin and evaluated their molluscicidal activities. This is with a view to providing information on development of useable particulate formulations for the control of snail intermediate hosts of schistosomiasis. The plant material was extracted successively in n-hexane (100%), chloroform (100%), chloroform/methanol (1:1) and methanol (100%) respectively by maceration. Bioactivity-guided fractionation of chloroform methanol extract was performed using column chromatography to isolate the active compound, Aridanin. Particulate formulations of chloroform methanol extract and Aridanin were prepared by emulsion solvent evaporation method. Molluscicidal screening on adult and newly hatched Biomphalaria glabrata snails was evaluated according to WHO protocols. The blank polymer formulation, dechlorinated water and Niclosamide served as controls. The result showed that Aridanin demonstrated the highest molluscicidal activity on adult B. glabrata snails with 24 h- LC50 of 1.61 ppm. The chloroform methanol extract had an LC50 value of 23.00 ppm, while particulate formulation of Aridanin and chloroform methanol extract had LC50 of 13.40 and 170.60 ppm respectively. This study showed that newly hatched B. glabrata snails are less susceptible to molluscicides compared to adult snails (P<0.05). Activities were concentration dependent. This study concluded that the developed particulate formulation of chloroform methanol extract and Aridanin is useful in snail control and have the potential for continuous release of the molluscicides, thereby reducing the need for repeated application of the molluscicides.

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References

[1] Makaula, P., Sadalaki, J. R., Muula, A. S., Kayuni, S., Jemu, S. and Bloch, P. (2014). Schistosomiasis in Malawi: a systematic review. Parasites and Vectors, 7: 570.
[2} Adenowo, A. F., Oyinloye, B. E., Ogunyinka, B. I. and Kappo, A. P. (2015). Impact of human schistosomiasis in sub-Saharan Africa. Brazilian Journal of Infectious Diseases, 19(2):196-205.
[3] Fenwick, A., Keiser, J. and Utzinger, J. (2006). Epidemiology, burden and control of schistosomiasis with particular consideration to past and current treatment trends. Drugs Future, 31: 413-425.
[4] WHO, (2010). Report of a Meeting to Review the Results of Studies on the Treatment of Schistosomiasis in Pre-school aged children, 1–23, World Health Organization.
[5] Utzinger, J., Raso, G. and Brooker, S. (2009). Schistosomiasis and neglected tropical diseases: towards integrated and sustainable control and a word of caution. Parasitology. 136: 1859–74.
[6] Gryseels, B., Polman, K., Clerinx, J. and Kestens, L. (2006). Human schistosomiasis. The Lancet, 368: 1106–1118.
[7] Massoud, A. M. and Habib, F. S. (2003). The effects of Myrrh Commiphora molmol on the infected snails of Schistosoma sp. and their egg masses: effect on shedding of cercariae and on snail fecundity. Journal of the Egyptian Society of Parasitology, 33(2): 585–596.
[8] Salawu, O. T. and Odaibo, A. B. (2011). The molliscicidal effects of Hyptis suaveolens on different stages of Bulinus globosus in the laboratory. African Journal of Biotechnology, 10(50): 10241–10247.
[9] Silva, L., Souza, B., De Almeida Bessa, E. C. and Pinheiro, J. (2012). Effect of successive applications of the sublethal concentration of Solanum paniculatum in Subulina octona (Subulinidae). Journal of Natural Products, 5: 157–167.
[10] Kenawy, E., and Rizk, E. (2004). Polymeric controlled release formulations of niclosamide for control of 422 Biomphalaria alexandrina, the vector snail of schistosomiasis. Macromolecular Bioscience, 4: 119-128.
[11] Nayaka, A. P., Tiyaboonchai, W., Patankar, S., Madhusudhan, B. and Souto, E. B. (2010). Curcuminoids-loaded lipid nanoparticles: a novel approach towards malaria treatment. Colloids and Surfaces B, 81: 263–273.
[12] Frezza, T. F., Gremião, M. P., Zanotti-Magalhães, E. M., Magalhães, L. A., Ribeiro de Souza, A. L. and Allegretti, S. M. (2013). Liposomal-praziquantel: Efficacy against Schistosoma mansoni in a preclinical assay. Acta Tropica, 128: 70–75.
[13] Oh, Y. J., Lee, J., Seo, J. Y., Rhim, T., Kim, S., Yoon, H. J. and Lee, K. Y. (2011). Preparation of budesonide-loaded porous PLGA microparticles and their therapeutic efficacy in a murine asthma model. Journal of Controlled Release, 150: 56–62.
[14] Pilcer, G. and Amighi, K. (2010). Formulation strategy and use of excipients in pulmonary drug delivery. International Journal of Pharmaceutics; 392: 1–19.
[15] Zhang, H., Cui, W., Bei, J. and Wang, S. (2006). Preparation of poly (lactide-co-glycolide-co-caprolactone) nanoparticles and their degradation behaviour in aqueous solution. Polymer Degradation and Stability, 91 (9): 1929-1936.
[16] Soppimath, K., Aminabhavi, T. and Kulkarni, A. (2001). Biodegradable polymeric nanoparticles as drug delivery devices. Journal of Controlled Release, 70: 1.
[17] Prombutara, P., Kulwatthanasal, Y., Supaka, N., Sramala, I. and Chareonpornwattana, S. (2012). Production of nisinloaded solid lipid nanoparticles for sustained antimicrobial activity. Food Control, 24: 184–190.
[18] Adewunmi, C. O., Adesina, S. K. and Marquis, V. O. (1982). Laboratory and field evaluation of the molluscicidal properties of Tetrapleura tetraptera. Bulletin of Animal Health and Production in Africa, 30: 89–94.
[19] Aladesanmi, J. A. (2007). Tetrapleura tetraptera: Molluscidal Activity and Chemical Constituents. African Journal of Traditional, Complementary and Alternative Medicine, 4 (1): 23–36.
[20] Liang, Y. S., Bruce, J. I. and Boyd, D. A. (1987). Laboratory cultivation of schistosome vector snails and maintenance of schistosome life cycle. Proceedings of the First Sino-American Symposium, 1: 34-48.
[21] WHO, (1983). Reports of the scientific group on plant molluscicide. Bulletin of the World Health Organization, 61(6): 927-929.
[22] Finney, D.J. (1971). Probit Analysis, 3rd ed. Cambridge University Press, Cambridge, 61-137.
[23] Mello-Silva, C. C., Vasconcellos, M. C., Pinheiro, J. and Rodrigues, M. L. (2006). Physiological changes in Biomphalaria glabrata Say, 1818 (Pulmonata: Planorbidae) caused by sub-lethal concentrations of the latex of Euphorbia splendens var. hislopii N.E.B (Euphorbiaceae). Memórias do Instituto Oswaldo Cruz, 101: 3-8.
[24] Bezerra, J. C., Silva, I. A., Ferreira, H. D., Ferri, P. H. and Santos, S. C. (2002). Molluscicidal activity against Biomphalaria glabrata of Brazilian Cerrado medicinal plants. Fitoterapia, 73: 428-430.
[25] Cantanhede, S. P., Marques, A. M., Silva-Souza, N. and Valverde, A. L. (2010). Atividade moluscicida de plantas: uma alternativa profilática. Brazilian Journal of Pharmacognosy, 20: 282-286.
[26] Lopes, T. C., Gonçalves, J. R., Souza, N. S., Moraes, D. F., Amaral, F. M. and Rosa, I. G (2011). Avaliação moluscicida e perfil fitoquímico das folhas de Caryocar brasiliensi Camb. Cadernos de Pesquisa, 18: 23-30.
[27] Adewunmi, C. O. (1984). Water extract of Tetrapleura tetraptera. An effective molluscicide for the control of Schistosomiasis and fascioliasis in Nigeria. Journal of Animal Production Research, 4(1): 73–84.
[28] Schenkel, E. P, Gosmann, G. and Athayde, M. L. (2004). Farmacognosia da planta ao medica-mento, 5ª ed., Editora da Universidade Federal do Rio Grande do Sul, 1102.
[29] Otarigho, B. and Morenikeji, O. A. (2012). Molluscicidal effects of aqueous and ethanolic extracts of lemongrass (Cymbopogon citratus) leaf against the different developmental stages of Biomphalaria pfeifferi. New York Science Journal, 5(8): 70–77.
[30] Mott, K. E. (1987). Plant molluscicides, UNDP/World Bank/WHO, John Wiley and Sons Ltd, New York 326.
[31] Badu M., John K. and Nathaniel O. (2012). Antioxidant activity of methanol and ethanol/water extracts of Tetrapleura tetraptera and Parkia biglobosa. International Journal of Pharmacy and Biological Sciences, 3(3): 312–321.
[32] DeSouza, C. P., Mendes, N. M., Araujo, N. and Katz, N. (1987). Molluscicidal activity of butanol extracts of Phytolacca dodecandra (endod) on Biomphalaria glabrata. Memórias do Instituto Oswaldo Cruz, 82: 345-349.
[33] Adewunmi, C.O. (1991). Plant molluscicides: Potential of aridanin from Tetrapleura tetraptera for schistosomiasis control in Nigeria. The Science of the Total Environment, 103: 21 – 23.
[34] Adesina, S. K., Adewunmi, C. O. and Marquis V. O. (1980). Phytochemical investigations of the molluscicidal properties of Tetrapleura tetraptera (Taub.). Journal of African Medicinal Plants, 3: 7-15.
Published
2019-09-09
How to Cite
Akinniyi, G., Fisusi, F., & Taiwo, B. (2019). Molluscicidal activities of Tetrapleura tetraptera fruit extract, Aridanin and their particulate formulations on adult and newly hatched Biomphalaria glabrata snails. Journal of Tropical Pharmacy and Chemistry, 4(6), 281-297. https://doi.org/10.25026/jtpc.v4i6.217