Antibacterial Activity of Scopoletin from Stem Bark of Aleurites moluccana Against Salmonella typhi

  • Wisnu Cahyo Prabowo Pharmacy
  • Risna Agustina
Keywords: Aleurites moluccana stem bark, KLT Bioautography, Salmonella Thypimurium, 7-hydroxy-6-methoxycoumarin

Abstract

Background: Based on a previous survey of ethnopharmaceutical studies (local culture), it was known that the use of decoction of candlenut stem bark (Aleurites Moluccana) was used as typhoid medicine in the treatment tradition of the people of East Kalimantan. In antibacterial screening by diffusion test, methanol extract of stem bark A.moluccana showed excellent growth inhibition of Salmonella thypimurium. Working method: Simplicia of A.moluccana stem bark was extracted by maceration using methanol. Maserate was extracted liquid-liquid using n-hexane and ethyl acetate solvents. The hexane and ethyl acetate fraction was monitored for active spots with TLC bioautography. The active compound is separated using vacuum liquid chromatography and radial chromatography. Results: The fraction J of KCV and J2 subfraction results from radial chromatography provides antibacterial activity of S. thpimurium. From the J2 subfraction obtained pure isolates in the form of yellowish needle crystals. The isolate was tested for antibacterial S.thypimurium using the microdilution method with a value of MIC is 250 µg/ml. Based on spectroscopic data and comparing the published spectra of the compound, the elucidation of the isolate is Scopoletin (7-hydroxy-6-methoxycoumarin).

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References

1. Acharya, D., Bogati, B. and Risal, P. (2013) ‘Scopoletin reduces intracellular survival of Salmonella typhi within U937 human macrophage cell line in vitro’, Sky Journal of Microbiology Research, 1(6), Hal. 47–51.
2. CLSI. 2012. Methods for Dilution Antimicrobial Susceptibility Tests for Bacteria that Grow Aerobically; Approved Standard-Ninth Edition. CLSI document M07-A9. Wayne, PA: Clinical and Laboratory Standards Institute.
3. Hart.T. 1997. Atlas Berwarna Mikrobiologi Kedokteran . Hipokrates. Ohio: 112-113
4. John A Crump. 2004. The Global Burden of Typhoid Fever. Bulletin of The World Health Organization. Atlanta, USA. Hal: 346-353
5. Leah Harvey. 2012. Typhoid in Indonesia, Surveillance and Policy Update, Ministry of Health Republic of Indonesia. Immunization Technical Advisory Group (ITAG) WHO/SEARO, Sabin Vaccine Institute
6. Li, C. et al. (2015) ‘Effect of Scopoletin on Apoptosis and Cell Cycle Arrest in Human Prostate Cancer Cells In vitro’, Tropical Journal of Pharmaceutical Research, 14(4), p. 611. doi: 10.4314/tjpr.v14i4.8.
7. Prabowo Wisnu Cahyo, Wirasutisna Komar Ruslan, Insanu Muhamad. 2013. Isolation and Characterization Of 3-Acetyl Aleuritolic Acid and Scopoletin from Stem Bark of Aleurites Moluccana (L.) Wild, International Journal of Pharmacy and Pharmaceutical Sciences, Vol. 5, Issue 3, Hal: 851-853.
8. Seo, E. J. et al. (2016) ‘Pharmacogenomics of scopoletin in tumor cells’, Molecules, 21(4). doi: 10.3390/molecules21040496.
9. De Souza, S. M., Delle Monache, F. and Smânia, A. (2005) ‘Antibacterial activity of coumarins’, Zeitschrift fur Naturforschung - Section C Journal of Biosciences, 60(9–10), Hal. 693–700.
10. Verma, A. et al. (2013) ‘Hypoglycemic and hypolipidemic activity of scopoletin (coumarin derivative) in streptozotocin induced diabetic rats’, International Journal of Pharmaceutical Sciences Review and Research, 22(1), Hal. 79–83
11. Wiart Christophe.2006. Medicinal Plants of The Asia-Pacific, Drugs for The Future. World Scientific Publishing Co.Pte.Ltd. Singapore: 337
Published
2020-06-30
How to Cite
Prabowo, W. C., & Agustina, R. (2020). Antibacterial Activity of Scopoletin from Stem Bark of Aleurites moluccana Against Salmonella typhi . Journal of Tropical Pharmacy and Chemistry, 5(1), 29-32. https://doi.org/10.25026/jtpc.v5i1.218