Antibacterial activity of different fractional extracts of cerastium glomeratum

  • Himayat Ullah Govt. Post Graduate College Mardan, kp, Pakistan
  • Muhammad Hubaib
  • Muhammad Israr
  • Muhammad Mushtaq
  • Muhammad Zeeshan
  • Muhammad Mustafa
Keywords: Antibacterial activity, different fractions of extract, well diffusion, S.aureus and E.coli


Every plant available in the universe is not produced to be the part of biodiversity, but is also produced to obtain medicinal purposes. The medicinal value of the plant is due to the availability of various chemical compounds [1]. Since prehistoric time, the crude extract of plant is used to cure various diseases, although the biologically active components of the plant were unidentified [2]. In the present study the antibacterial activity is performed for various extracts of cerastium glomeratum. The plant cerastium glomeratum was collected from near kalpani river Mardan, Pakistan and was identified by lecturer Israr department of botany GPGC Mardan, Pakistan. The plant was dried under shade and grinded into powder form. The extraction process was done by using soxhlet apparatus, reflux condenser and maceration process using ethanol as a solvent. The extract was divided into different fractions of n-hexane, DCM, ethyl acetate and water. These four extracts were tested against gram positive (staphylococcus aureus) and gram negative (E.coli) bacteria using well diffusion technique. The water extract show a wide range inhibition zone against both gram positive and gram negative bacteria while n-hexane, DCM and ethyl acetate fractions show no zone of inhibition against bacteria. In effort to find new antibacterial compounds cerastium glomeratum seems to be a good plant for additional phytochemical studies.


Download data is not yet available.


. Plants as a Source of Medicine Patel DK* Rural Technology Department, Guru Ghasidas Vishwavidyalaya (A Central University) – Bilaspur (Chhattisgarh), 495009,
2. Economic and medicinal properties of some medicinal plants found in Kashmir Himalaya
Shagoon Khan, Azra Kamili, Raghuir Chand Gupta.
3. F. Ullah, M. Zafar, M. Amhad, S. Sultana, A. Ullah, S. N. Shah and S. Mir, Flora 242, 31–44 (2018).
4. A. Perveen and M. Qaiser, Pollen flora of Pakistan-LI-Caryophyllaceae Pakistan Journal of Botany, 38(4), 901(2006)
5. S.A. Ghazanfar, and Y.J. Nasir, Caryophyllaceae. In: Flora of Pakistan. (Eds.): E. Nasir and S. I. Ali., Dept. Bot. Univ. Karachi and National Herbarium (Stewart Coll.) Pak Agri. Research council, Islamabad No. 175 (1986)
6. Yu. D. Gusev and Yu. P. Kozhevnikov plants of central Asia plant collections from China and Mongolia (Ed. Grubov V.I.) Amaranthaceae—Caryophyllaceae Science Publishers, Enfield, NH, USA Volume 11(2007)
7. D. J. Mabberley, Mabberley’s plant-book: a portable dictionary of plants, their classifications, and uses. Cambridge: Cambridge University Press 1040–1(2008)
8. Ghazanfar, S.A. and Nasir, Y.J. (1986) Caryophyllaceae. No. 175 In: Flora of Pakistan. (Eds.): E. Nasir and S. I. Ali. Dept. Bot. Univ. Karachi and National Herbarium, (Stewart Coll.) Pak Agri. Research conucil, Islamabad.

9. N.Z. Mamadalieva, R. Lafont and M. Wink, Diversity of secondary metabolites in the genus Silene L. (Caryophyllaceae) structures, distribution, and biological properties, Diversity, 6,415-499 (2014)
10. L. Zibareva, V. Volodin, Z, Saatov, T. Savchenko, Whiting, R. Lafont and L. Dinan, Distrubition of phytoecdysteroids in the Caryophyllaceae, Phytochemistry, 64, 499-517 (2003)
11. Z. Jia, K. Koike, N.P. Sahu and T. Nikaido, Triterpenoid saponins from Caryophyllaceae family, Atta-ur-Rahman (Ed.) Studies in Natural Products Chemistry, 26, 3-6 (2002)
12. Y. Meng, P. Whiting, L. Zibareva, G. Bertho, J. P. Girault, R. Lafont, and L. Dinan, Identification and quantitative analysis of the phytoecdysteroids in Silene species (Caryophyllaceae) by high-performance liquid chromatography Novel ecdysteroids from S. pseudotites, Journal of Chromatography A, 935, 309–319 (2001)
13. F. Galeotti, E. Barile, P, Curir M, Dolci and V. Lanzotti, Flavonoids from carnation Dianthus caryophyllus and their antifungal activity. Phytochem Lett 1, 44–8 (2008)
14. T. Asai, Y. Nakamura, Y. Hirayama, K. Ohyama, Y. Fujimoto, Cyclic glycolipids from glandular trichome exudates of Cerastium glomeratum Phytochemistry 82, 149-157 (October 2012)
15. G. R. Jamieson and E. H. Reid, The leaf lipids of some members of the Caryophyllaceae Phytochemistry 10, 1575–1577 (1971).
16. N. P. Manandhar, Plants and People of Nepal Timber Press. Oregon. ISBN 0-88192-527-6 (2002)
17. Manandhar. N. P. (2002) Plants and People of Nepal Timber Press. Oregon.
ISBN 0-88192-527-6.
How to Cite
Himayat Ullah, Muhammad Hubaib, Muhammad Israr, Muhammad Mushtaq, Muhammad Zeeshan, & Muhammad Mustafa. (2020). Antibacterial activity of different fractional extracts of cerastium glomeratum. Journal of Tropical Pharmacy and Chemistry, 5(2), 57-62.

Most read articles by the same author(s)