The Effectiveness of Sea Urchin Extract (Echinometra matthaei) for Wound Healing on Deep Second-Degree in White Rats (Rattus norvegicus) Wistar

  • Angelica Kresnamurti Program Studi Farmasi, Universitas Hangtuah, Surabaya
  • Dita Nurlita R Program Studi Farmasi, Universitas Hangtuah, Surabaya
  • Fitri Budiarti Universitas Hangtuah
Keywords: Echinometra matthaei, burns, silversulfadiazine, wound healing, napthoquinone

Abstract

Background: Burns is a form of tissue damage caused by high temperatures. Echinometra matthaei sea urchins have several secondary metabolites that can potentially help in the healing process of burns. In this study, 70% E. matthaei ethanol extract was formulated in the form of O / W (oil in water) type cream preparations which were applied topically. Objective: This study aims to determine the effectiveness of E. matthaei ethanol extract cream preparations on the healing of second-degree burns in Wistar strain rats. In this study preparations were made in 3 formulations, namely Formulation 1 (extract concentration of 1%), Formulation 2 (extract concentration of 3%), and Formulation 3 (extract concentration of 5%). This research was conducted for 7 days with the method used is the post-test only control group design. Experiments were given induction of burns using a hot plate with a diameter of 20 mm at a temperature of ± 200ºC for 15 seconds. Wound healing is observed periodically by observing macroscopically healing inflammation of the inflammatory phase and observing tissue growth in the proliferation phase. Results: The average percentage of inflammation healing showed improvement in the F2: 100%, F1: 80%, F3: 71% results were better than the wound group: 50% as evidenced by the value α = 0.012 (<0.05) means there are significant differences. Conclusion: The conclusion of this study shows that the ethanol extract of E. matthaei made in cream preparations has the effectiveness of healing of seconddegree burns with a formulation of 3% is best formulation

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References

[1] Aprilia H.A, Pringgenies D, & Yudiati E, 2012. Uji Toksisitas Ekstrak Kloroform Cangkang dan Duri Landak Laut (Diadema setosum) Terhadap Mortalitas Nauplius artemia sp, Journal of Marine Research, 1(1),75-83. https://dx.doi.org/10.14710/jmr.v1i1.890. [2] Dahl W.J, Jebson P, & Louis D.S, 2010. Sea Urchin Injuries to The Hand: A Case Report and Review of The Literature, The lowa Orthopaedic Journal, (30),153-156. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2958287/. [3] Shankarlal S. K. Prabu, & E. Natarajan, 2011. Antimicrobial and Antioxidant Activity of Purple Sea Urchin Shell (Salmacis virgulata L. Agassiz and Desor 1846), American-Eurasian Journal of Scientific Research 6, (3): 178-181. https://www.semanticscholar.org/paper/Antimicrobial-and-Antioxidant-Activity-of-PurpleL.-Shankarlal-Prabu/aac22adb623a05281032c4583e2e7123e43175e4. [4] Moenadjat Y, 2009. Masalah Luka Bakar dan Tata Laksana, Balai Penerbit, FK UI, Jakarta. [5] Guo S, & L. A. DiPietro, 2010. Factors Affecting Wound Healing, Departement of Periodontics, J Dent Res 89(3):219-229, USA. DOI: 10.1177/0022034509359125. [6] N. A. Patel et al, 2011. Formulation and Evaluation of Polyherbal Gel for Wound Healing, International Research Journal of Pharmaceuticals, Vol. 01, Issue 01, pp. 15-20. https://www.researchgate.net/publication/268422819_Formulation_and_Evaluation_of_Pol yherbal_Gel_for_Wound_Healing. [7] N. S. Jagtap et al, 2009. Development and Evaluation of Herbal Wound Healing Formulations. International Journal of PharmTech Research, Vol.1, No.4, pp 1104-1108. https://www.academia.edu/15139190/DEVELOPMENT_AND_EVALUATION_OF_HER BAL_WOUND_HEALING_FORMULATIONS [8] Akhoondinasab Mohammad Reza, Motahhare Akhoondinasab, Mohsen Saberi, 2014. Comparison of Healing Effect of Aloe Vera Extract and Silversulfadiazine in Burn Injuries in Experimental Rat Model, Original Article, Vol. 3. No.1: 29-34. https://www.researchgate.net/publication/269413849_Comparison_of_Healing_Effect_of_ Aloe_Vera_Extract_and_Silver_Sulfadiazine_in_Burn_Injuries_in_Experimental_Rat_Mo del [9] Fatmawaty A, T Apolarosa R, Rhadia, & Michrun N, 2012. Formulasi, Evaluasi Fisik dan Permeasi Krim Pemutih Asam Kojat dengan Variasi Enhancer, Majalah Farmasi dan Farmakologi, Vol.16 No.3: 139-142. https://www.e-jurnal.com/2013/11/formulasievaluasi-fisik-dan-permeasi.html [10] Indrayudha P, Dewi Rahmawati, Anita Sukmawati, 2010. Formulasi Krim Minyak Atsiri Rimpang Temu Giring (Curcuma heyneana Val & Zijp): Uji Sifat Fisik Dan Daya Antijamur Terhadap Candida albicans Secara in Vitro, Majalah Obat Tradisional, 15(2),56-63. https://www.researchgate.net/publication/260158609_FORMULASI_KRIM_MINYAK_A TSIRI_RIMPANG_TEMU_GIRING_Curcuma_heyneana_Val_Zijp_UJI_SIFAT_FISIK_ DAN_DAYA_ANTIJAMUR_TERHADAP_Candida_albicans_SECARA_IN_VITRO_FO RMULATION_CREAM_CONTAINING_ESSENTIAL_OIL_OF_Cu
[11] Rowe R. C, Sheskey P. J, Quinn M. E, 2009. Handbook of Pharmaceutical Excipients 6th edition, London; Pharmaceutical Press. [12] Nurdianti Lusi, Ira Rahmiyani, 2016. Uji Aktivitas Antioksidan Krim Ekstrak Daun Mangga (Mangifera indica) Terhadap DPPH (1,1-diphenyl-2-picrylhydrazil), Jurnal Kesehatan Bakti Tunas Husada, Vol. 16,No.1.(Doi: http://dx.doi.org/10.36465/jkbth.v16i1.165). [13] Syuhar MN, Windarti I, Kurniawati E, 2015. The Comparison of Second Degree Burns Healing Rate Between the Smear of Honey And The Collision of Binahong Leaves In Sprague Dawley Rats, Journal Majority, Vol.6 No.1, 2015:103–12. https://docplayer.info/44711389-The-comparison-of-second-degree-burns-healing-ratebetween-the-smear-of-honey-and-the-collision-of-binahong-leaves-in-sprague-dawleyrats.html [14] Aziz, Zoriah, and Bassman Abdul Rasool Hasan, 2017. The Effect of Honey Compared to Silver Sulfadiazine for The Treatment of Burns: a Systematics Review of Randomized Controlled Trials, Burns, 43.1: 50-57. doi: 10.1016/j.burns.2016.07.004. [15] Venkataraman M, & Nagarsenker M, 2013. Silver Sulfadiazine Nanosystems for Burn Therapy, AAPS PharmSciTech, 14 (1):254-64. doi: 10.1208/s12249-012-9914-0 [16] Mardiyantoro Fredy, Khusnul Munika, Viranda Sutanti, Miftakhul Cahyati, Ariyati Retno Pratiwi, 2018. Penyembuhan Luka Rongga Mulut. Malang; UB Press. [17] Politis C, Schoenaers, Jacobs R and Agbaj J O, 2016. Wound Healing Problems in the Mouth, Frontiers in Physiology 7, Doi: 10.3389/fphys.2016.00507. doi: 10.3389/fphys.2016.00507 [18] Castro M. A, Gamito A. M, Tangarive-Castano V, Zapata B et al, 2013. Synthesis Antifungal Activity of Terpenyl-1,4 naphthoquinone and 1,4 Anthracenedione Derivatives, Eur J Med Chem, 67: 19-27. https://doi.org/10.1016/j.ejmech.2013.06.018 [19] Kumagai Y, Shinkai Y, Miura T & Cho A K, 2012. The Chemical Biology of Naphthoquinones and Its Environmental Implications, Annu Rev Pharmacol Toxicol, 52: 221-247. DOI: 10.1146/annurev-pharmtox-010611-134517 [20] M. T Simanjuntak, 2006. Biofarmasi Sediaan Yang Diberikan Melalui Kulit, USU Repository. [21] Aponno, Jeanly V, Paulina V. Y. Yamlean, Hamidah S. S, 2014. Uji Efektivitas Sediaan Gel Ekstrak Etanol Daun Jambu Biji (Psidium guajava) Terhadap Penyembuhan Luka Yang Terinfeksi Bakteri Staphylococcus aureus Pada Kelinci (Oryctolagus cuniculus), PHARMACON Jurnal Ilmiah Farmasi-UNSRAT 3 (3): 2302-2493. DOI: https://doi.org/10.35799/pha.3.2014.5400 [22] Lostapa IWFW, Warditha AAGJ, Pemayun IGAGP, Sudimartini NLM, 2015. Kecepatan Kesembuhan Luka Insisi Yang Diberi Amoksisilin Dan Asam Mefenamat Pada Tikus Putih, Bul. Vet. Udayana, 8(2): 172-179. https://www.semanticscholar.org/paper/KecepatanKesembuhan-Luka-Insisi-Yang-Diberi-Dan-LostapaWardhita/83aea189c69e6aa8d2a0fd0a9180bf8d0cf715e7
[23] Kimura Y, Sumiyoshi M, Kawahira K, and Sakanaka M, 2006. Effects of Ginseng Saponins Isolated from Red Ginseng Roots on Burn Wound Healing in Mice, British Journal of Pharmacology, 148: 860-870. DOI: 10.1038/sj.bjp.0706794 [24] Junqueira L. C, Carneiro J, Contopoulos A. N, 2007. Basic Histology, 10th ed, Lange Publications, p.82, Los Altos.
Published
2021-04-03
How to Cite
Kresnamurti, A., Nurlita R, D., & Budiarti, F. (2021). The Effectiveness of Sea Urchin Extract (Echinometra matthaei) for Wound Healing on Deep Second-Degree in White Rats (Rattus norvegicus) Wistar. Journal of Tropical Pharmacy and Chemistry, 5(3), 194-202. https://doi.org/10.25026/jtpc.v5i3.281