Analysis of Ethyl p-Methoxycinnamate from Kaempferia galanga L. Extract by High Performance Liquid Chromatography

Authors

  • Wiwin Winingsih Sekolah tinggi Farmasi Indonesia Bandung
  • Sri Gustini Husein Sekolah Tinggi Farmasi Indonesia (STFI) Jl. Soekarno-Hatta No.354 (Parakan Resik 1), Bandung https://orcid.org/0000-0003-2081-3183
  • Rozalia Putri Neno Ramdhani Sekolah Tinggi Farmasi Indonesia (STFI) Jl. Soekarno-Hatta No.354 (Parakan Resik 1), Bandung

DOI:

https://doi.org/10.25026/jtpc.v5i4.331

Keywords:

Ethyl para-methoxycinamate, high performance liquid chromatography, Kaempferia galanga L.

Abstract

Ethyl para-methoxycinamate (EPMS) is a major compound of Kaempferia galanga L that has anti-inflammatory effect.  The purpose of this study was to determine of EPMS in Kaempferiae galanga L rhizome extract by  High Performance Liquid Chromatography (HPLC) and evaluated the performance of the analysis. This study included determination of system suitability, accuracy, precision, linearity and range, limit of detection (LOD) and Limit of quantitation (LOQ) and selectivity.  The results of system suitability test  HPLC System for EPMS analysis were as follows isocratic elution system of a mobile phase mixture of methanol: water (70:30) containing 0.1% TFA, uv detector at a wavelength of 308 nm using column C18 (150 × 4, 6mm, 5?m) flow rate 1 ml / min. From the analysis, it was found that the average EPMS content was 78.74%. Then method had linear concentration range from 5-360 ppm, with R ² = 0.9999. The LOD and LOQ were 7.0722 ppm and 21.4311 ppm respectively. The accuracy of this method that represented by % recovery was 98.02% - 101.26%. The precision of this method that expressed by Relative Standard Deviation (RSD) was 1.57%. The selectivity of this method that showed by  resolution value was 2.6. Based on the results of the system suitability test and analysis performance evaluation,all parameters met the requirements.

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References

[1] Ismiarni Komala, Supandi , Nurhasni , Ofa Suzanti Betha , Eka Putri , Syarifatul Mufidah , Muhammad Fikry Awaludin , Mida Fahmi , Muhammad Reza , and Nurkhayati Putri Indriyani, 2018, Structure-Activity Relationship Study on the Ethyl p-Methoxycinnamate as an Anti-Inflammatory Agent, Indones. J. Chem., 18 (1), 60 - 65,
[2] Muhammad Ihtisham Umar , Mohd Zaini Asmawi, Amirin Sadikun, Item J Atangwho, Mun Fei Yam, Rabia Altaf, Ashfaq Ahmed, 2012, Bioactivity-guided isolation of ethyl-p-methoxycinnamate, an anti-inflammatory constituent, from Kaempferia galanga L. extracts, Molecules, 17(7), 8720-34.
[3] Nupur Srivastava, Ranjana, Shilpi Singh, Amit Chand Gupta, Karuna Shanker, Dnyaneshwar U. Bawankule, and Suaib Luqman, 2019, Aromatic ginger (Kaempferia galanga L.) extracts with ameliorative and protective potential as a functional food, beyond its flavor and nutritional benefits, Toxicol. Rep. , 6, 521–528.
[4] S. Kavita, S. Rahul, S. Ashish, 2014, HPLC method for standardization of Hedychium spicatum (Ham) oil using EPMC as a marker compound, Chemistry
[5] Arslan Azizoglu, Gulcin Azizoglu, Erkan Tuncay Tanriverdi, Sakine Ozer, Ozgen, 2017, Marmara Pharmaceutical Journal,21(4), 921-930.
[6] Meenakshi S. Akhade, Poonam A. Agrawal, and K. S. Laddha, 2013, Development and Validation of RP-HPLC Method for Simultaneous Estimation of Picroside I, Plumbagin, and Z-guggulsterone in Tablet Formulation, Indian J Pharm Sci., 75(4), 476–482.
[7] Sari, P., Fatimawali., dan Runtuwene, J,R, 2014, Validasi Metode Analisis Kromatografi Cair Kinerja Tinggi Untuk Penetapan Kadar Amoxicilin Dalam Plasma secara In Vitro, Jurnal Ilmiah Farmasi , 4 (3), 96-103.
[8] Kui Zhang, Wenxia Wu, Shuge Tian, 2020, Comparative study on the determination of ethyl p-methoxycinnamate in Kaempferia galanga rhizome by HPTLCS and HPLC, JPC – Journal of Planar Chromatography – Modern TLC, 33, 51–57.
[9] Andrea N. de L. Batista, Renata Colombo, Inara C. de Pascoli, Helder L. Teles, Geraldo H. Silva, Giovanni C. C. Bomfim, Rosilene C. R. Burgos, Alberto J. Cavalheiro, Vanderlan da Silva Bolzani, Dulce H. S. Silva, Maria Célia H. Reimberg, 2011, Development and validation of a HPLC method for standardization of herbal and commercial extracts of Myrcia uniflora, Rev. bras. Farmacogn, 21(3).
[10] Pratik J. Purohit, Pankaj P. Kapupara, Ketan V. Shah, 2014, Development and Validation of Analytical Method for Simultaneous Estimation of Curcumin and Gallic Acid in Different Polyherbal Formulations by HPLC, Research J. Pharm. and Tech., 7(7),749-753.

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Published

2021-12-31

How to Cite

Winingsih, W., Husein, S. G., & Ramdhani, R. P. N. (2021). Analysis of Ethyl p-Methoxycinnamate from Kaempferia galanga L. Extract by High Performance Liquid Chromatography. Journal of Tropical Pharmacy and Chemistry, 5(4), 353–358. https://doi.org/10.25026/jtpc.v5i4.331