Isolation and Evaluation of Antibacterial Potential Test of Plant Carthamus oxycantha

  • Amir Hassan Department of Chemistry, Government Post Graduate College Mardan, Abdul Wali Khan University, Mardan 23200 Khyber Pakhtunkhwa, Pakistan
  • Ibad Ullah Department of Botany, Government Post Graduate College Mardan, Abdul Wali Khan University, Mardan 23200 Khyber Pakhtunkhwa, Pakistan
  • Waqas Ahmad Department of Botany, Government Post Graduate College Mardan, Abdul Wali Khan University, Mardan 23200 Khyber Pakhtunkhwa, Pakistan
Keywords: Carthamus oxycantha, Isolation, Antibacterial, Agar well diffusion, E-coli, S. aureus


The present investigation was initiated to find a suitable alternative to synthetic antibiotics for the management of diseases caused by bacteria. Carthamus oxycantha.L locally known as wild safflower member of family Asteraceae that grows wildly. The study was conducted using as Agar well diffusion to trace the antibacterial potential for to evaluate the efficiency of ethanolic extract of Carthamus oxycantha with concentration of 05, 10, 15, and 20 mg/ml against gram-positive Staphylococcus aureus and gram-negative Escherichia Coli species and them compared with that of Clindamycin, Ampicillin and Kanamycin (10 mg). Zone of inhibition for the extracts were 10.667 to 20.00 mm as compared to standard drug Clindamycin, Ampicillin and kanamycin (15.00-20.00 mm). Antibacterial assays indicates that Carthamus oxycantha has potential natural antimicrobial agents against E-coli and S. aureus. The findings of the present study suggested that ethanolic extract of C. oxycantha has strong potential to serve as possible antibacterial.


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Author Biographies

Amir Hassan, Department of Chemistry, Government Post Graduate College Mardan, Abdul Wali Khan University, Mardan 23200 Khyber Pakhtunkhwa, Pakistan

Department of Chemistry, Government Post Graduate College Mardan, Abdul Wali Khan University, Mardan 23200  Khyber Pakhtunkhwa, Pakistan

Ibad Ullah, Department of Botany, Government Post Graduate College Mardan, Abdul Wali Khan University, Mardan 23200 Khyber Pakhtunkhwa, Pakistan

Department of Botany, Government Post Graduate College Mardan, Abdul Wali Khan University, Mardan 23200      Khyber Pakhtunkhwa, Pakistan

Waqas Ahmad , Department of Botany, Government Post Graduate College Mardan, Abdul Wali Khan University, Mardan 23200 Khyber Pakhtunkhwa, Pakistan

Department of Botany, Government Post Graduate College Mardan, Abdul Wali Khan University, Mardan 23200 Khyber Pakhtunkhwa, Pakistan


1. Srivastava, J.; Lambert, J.; Vietmeyer, N., Medicinal plants: An expanding role in development. World Bank Publications: 1996; Vol. 320.
2. Zhang, X.; Organization, W. H., Traditional medicine strategy 2002 2005. 2002.
3. Suffredini, I.; Sader, H. S.; Gonçalves, A. G.; Reis, A. O.; Gales, A. C.; Varella, A. D.; Younes, R. N., Screening of antibacterial extracts from plants native to the Brazilian Amazon Rain Forest and Atlantic Forest. Brazilian journal of medical and biological research 2004, 37 (3), 379-384.
4. Edeoga, H. O.; Okwu, D.; Mbaebie, B., Phytochemical constituents of some Nigerian medicinal plants. African journal of biotechnology 2005, 4 (7), 685-688.
5. Kaushik, R.; Garg, G.; Sharma, G.; Arora, C., Antibacterial activity of plant extracts from Uttaranchal Hills, India. Allelopathy Journal 2003, 12 (2), 205-214.
6. Hassan, A.; Akmal, Z.; Khan, N., The Phytochemical Screening and Antioxidants Potential of Schoenoplectus triqueter L. Palla. Journal of Chemistry 2020, 2020.
7. Hassan, A.; Ullah, H., Antibacterial and antifungal activities of the medicinal plant veronica biloba. Journal of Chemistry 2019, 2019.
8. Hassan, A., Traditional Chinese Herbal Medication for corona-virus 2019. Travel medicine and infectious disease 2020, 36, 101810.
9. Hassan, A.; Ashfaq, M.; Khan, A.; Khan, M. S., Isolation of Caffeine from Carbonated Beverages. Journal of Tropical Pharmacy and Chemistry 2020, 5 (1), 33-38.
10. Kafaru, E., Immense help formative workshop. Essential pharmacology 1994, 1.
11. Harbottle, H.; Thakur, S.; Zhao, S.; White, D., Genetics of antimicrobial resistance. Animal biotechnology 2006, 17 (2), 111-124.
12. Namita, P.; Mukesh, R., Medicinal plants used as antimicrobial agents: a review. Int Res J Pharm 2012, 3 (1), 31-40.
13. Dahiya, P.; Purkayastha, S., Phytochemical screening and antimicrobial potentials of Alangium salvifolium and Piper longum against multi-drug resistant bacteria from clinical isolates. International Journal of Pharmacy and Pharmaceutical Sciences 2011, 3 (5), 462-465.
14. Riazunnisa, K.; Adilakshmamma, U.; Khadri, C., Phytochemical analysis and in vitro antibacterial activity of Soymida febrifuga (Roxb.) Juss. and Hemidesmus indicus (L.). Indian J. Appl. Res 2013, 3, 57-59.
15. Awouafack, M. D.; McGaw, L. J.; Gottfried, S.; Mbouangouere, R.; Tane, P.; Spiteller, M.; Eloff, J. N., Antimicrobial activity and cytotoxicity of the ethanol extract, fractions and eight compounds isolated from Eriosema robustum (Fabaceae). BMC Complementary and Alternative Medicine 2013, 13 (1), 1-9.
16. Das, K.; Tiwari, R.; Shrivastava, D., Techniques for evaluation of medicinal plant products as antimicrobial agents: current methods and future trends. Journal of medicinal plants research 2010, 4 (2), 104-111.
17. Ali, M. N.; Khan, M. M., Antibacterial activity of Herbal and Spice Extracts on Bacterial Clinical Isolates of Urinary Tract Infections. Journal of Chemical, Biological and Physical Sciences (JCBPS) 2017, 7 (3), 718.
18. Bandaranayake, W., Traditional and medicinal uses of mangroves. Mangroves and salt marshes 1998, 2 (3), 133-148.
19. Group, S. f. M. o. A. T. S., CD4+ count–guided interruption of antiretroviral treatment. New England Journal of Medicine 2006, 355 (22), 2283-2296.
20. Nascimento, G. G.; Locatelli, J.; Freitas, P. C.; Silva, G. L., Antibacterial activity of plant extracts and phytochemicals on antibiotic-resistant bacteria. Brazilian journal of microbiology 2000, 31 (4), 247-256.
21. Rehman, S.; Hasnat, A.; Hasan, C.; Rashid, M.; Ilias, M., Pharmacological evaluation of Bangladeshi medicinal Plant—A Review. Pharmacol. Biol 2001, 37, 202-207.
22. Fabricant, D. S.; Farnsworth, N. R., The value of plants used in traditional medicine for drug discovery. Environmental health perspectives 2001, 109 (suppl 1), 69-75.
23. Mocan, A.; Vodnar, D.; Vlase, L.; Crișan, O.; Gheldiu, A.M.; Crișan, G. Phytochemical characterization of Veronica officinalis L., V. teucrium L. and V. orchidea Crantz from Romania and their antioxidant and antimicrobial properties. International journal of molecular sciences 2015, 16, 21109-21127,
24. Dunkic, V.; Kosalec, I.; Kosir, I.J.; Potocnik, T.; Cerenak, A.; Koncic, M.Z.; Vitali, D.; Muller, I.D.; Kopricanec, M.; Bezic, N. Antioxidant and antimicrobial properties of Veronica spicata L.(Plantaginaceae).Current drug targets 2015, 16, 1660-1670,
25. Sharifi-Rad, J.; Iriti, M.; Setzer, W.N.; Sharifi-Rad, M.; Roointan, A.; Salehi, B. Antiviral activity of Veronica persica Poir. on herpes virus infection. Cellular and Molecular Biology 2018, 64, 11-17,
26. Stojković, D.S.;Živković, J.; Soković, M.; Glamočlija, J.; Ferreira, I.C.;Janković, T.; Maksimović, Z. Antibacterial activity of Veronica montana L. extract and of protocatechuic acid incorporated in a food system.Food and Chemical Toxicology 2013, 55, 209-213,
27. Sharifi-Rad, J.; Tayeboon, G.S.; Niknam, F.; Sharifi-Rad, M.; Mohajeri, M.; Salehi, B.; Iriti, M.; Sharifi-Rad, M. Veronica persica Poir. extract–antibacterial, antifungal and scolicidal activities, and inhibitory potential on acetylcholinesterase, tyrosinase, lipoxygenase and xanthine oxidase. Cellular and Molecular Biology 2018, 64, 50-56,
28. Alabri, T.H.A.;Al Musalami, A.H.S.;Hossain, M.A.;Weli, A.M.;Al-Riyami, Q. Comparative study of phytochemical screening, antioxidant and antimicrobial capacities of fresh and dry leaves crude plant extracts of Datura metel L. Journal of King Saud University-Science 2014, 26, 237-243,
29. Alghazeer, R.; El-Saltani, H.; Saleh, N.; Al-Najjar, A.; Hebail, F. Antioxidant and antimicrobial properties of five medicinal Libyan plants extracts. Natural science 2012,4, 324,
30. Edziri, H.; Mastouri, M.; Cheraif, I.; Aouni, M. Chemical composition and antibacterial, antifungal and antioxidant activities of the flower oil of Retama raetam (Forssk.) Webb from Tunisia.Natural product research 2010, 24, 789-796,
31. Cushnie, T.T.; Lamb, A.J. Antimicrobial activity of flavonoids. International journal of antimicrobial agents 2005, 26, 343-356,
32. Hernández, N.E.; Tereschuk, M.; Abdala, L. Antimicrobial activity of flavonoids in medicinal plants from Tafı del Valle (Tucuman, Argentina).Journal of Ethnopharmacology 2000, 73, 317-322,
33. Epand, R.F.; Savage, P.B.; Epand, R.M. Bacterial lipid composition and the antimicrobial efficacy of cationic steroid compounds (Ce ragenins). Biochimica et Biophysica Acta (BBA)-Biomembranes 2007, 1768, 2500-2509,
34. Kumar, V. P.; Chauhan, N. S.; Padh, H.; Rajani, M., Search for antibacterial and antifungal agents from selected Indian medicinal plants. Journal of ethnopharmacology 2006, 107 (2), 182-188.
35. Koochak, H.; Seyyednejad, S. M.; Motamedi, H., Preliminary study on the antibacterial activity of some medicinal plants of Khuzestan (Iran). Asian Pacific Journal of Tropical Medicine 2010, 3 (3), 180-184.
36. Dwivedi, S.; Dudey, R.; Tyagi, R.; Masand, M.; Advani, U., Medicinal Bioactives as antimicrobial agents: an overview. Int. J. Pharm. Dev 2011, 3, 24-30.
37. Malesh, B.; Satish, S., Antimicrobial Activity of some important medicinal plant against plant and human pathogen. World Journal of Agriculture Sciences 2008, 4 (5), 839-843.
38. Sanyal, B.; Sharma, A. Cytological Studies in Indian Cyperaceae. Cytologia 1972, 37, 13-32,
39. D'abrosca, B.; Dellagreca, M.; Fiorentino, A.; Isidori, M.; Monaco, P.; Pacifico, S. Chemical constituents of the aquatic plant Schoenoplectus lacustris: evaluation of phytotoxic effects on the green alga Selenastrum capricornutum. Journal of chemical ecology 2006, 32, 81-96,
40. Liu, X.; Wang, Z.; Zhang, X.; Wang, J.; Xu, G.; Cao, Z.; Zhong, C.; Su, P. Degradation of diesel-originated pollutants in wetlands by Scirpus triqueter and microorganisms. Ecotoxicology and environmental safety 2011, 74, 1967-1972,
41. Jiménez‐Mejías, P.; Luceno, M.; Castroviejo, S. Schoenoplectus corymbosus: a tropical Old‐World sedge (Cyperaceae) discovered in Spain and Morocco. Nordic Journal of Botany 2007, 25, 70-74,
42. Roalson, E.H. A synopsis of chromosome number variation in the Cyperaceae. The Botanical Review 2008, 74, 209-393,
43. Chang, A.K.T.; Frias Jr, R.R.; Alvarez, L.V.; Bigol, U.G.; Guzman, J.P.M.D. Comparative antibacterial activity of commercial chitosan and chitosan extracted from Auricularia sp. Biocatalysis and agricultural biotechnology 2019, 17, 189-195,
44. de Aragao, A.P.; de Oliveira, T.M.; Quelemes, P.V.; Perfeito, M.L.G.; Araujo, M.C.; Santiago, J.D.A.S.; Cardoso, V.S.; Quaresma, P.; de Almeida, J.R.D.S.; da Silva, D.A. Green synthesis of silver nanoparticles using the seaweed Gracilaria birdiae and their antibacterial activity. Arabian Journal
of Chemistry 2019, 12, 4182-4188,
45. Gummuluri, S.; Kavalipurapu, V.T.; Kaligotla, A.V. Antimicrobial efficacy of Novel Ethanolic Extract of Morinda Citrifolia Against Enterococcus Feacalis by Agar Well Diffusion Method and Minimal Inhibitory Concentration-An Invitro Study. Brazilian Dental Science 2019, 22, 365-370,
46. Majumder, R.; Dhara, M.; Adhikari, L.; Ghosh, G.; Pattnaik, S. Evaluation of in vitro Antibacterial and Antioxidant Activity of Aqueous Extracts of Olax psittacorum. Indian Journal of Pharmaceutical Sciences 2019, 81, 99-109,
47. Raj, S.R.G.; Ng, C.H.; Mohd-Said, S.; Yu, K.X. Antibacterial Potential of Aqueous Extracts and Compounds from Selected Brown Seaweeds. INNOSC Theranostics and Pharmacological Sciences 2019, 2,
48. Tlili, H.; Marino, A.; Ginestra, G.; Cacciola, F.; Mondello, L.; Miceli, N.; Taviano, M. F.; Najjaa, H.; Nostro, A. Polyphenolic profile, antibacterial activity and brine shrimp toxicity of leaf extracts from six Tunisian spontaneous species. Natural product research 2019, 1-7,
49. Aghraz, A.; Albergamo, A.; Benameur, Q.; Salvo, A.; Larhsini, M.; Markouk, M.; Gervasi, T.; Cicero, N. Polyphenols contents, heavy metals analysis and in vitro antibacterial activity of extracts from Cladanthus arabicus and Bubonium imbricatum of Moroccan Origin. Natural product research 2020, 34, 63-70,
50. Fernández-Agulló, A.; Pereira, E.; Freire, M.S.; Valentao, P.; Andrade, P.; González-Álvarez, J.; Pereira, P. Influence of solvent on the antioxidant and antimicrobial properties of walnut (Juglans regia L.) green husk extracts.Industrial crops and products 2013, 42, 126-132,
51. Roby, M.H.H.;Sarhan,M.A.; Selim, K.A.H.; Khalel, K.I. Evaluation of antioxidant activity, total phenols and phenolic compounds in thyme (Thymus vulgaris L.), sage (Salvia officinalis L.), and marjoram (Origanum majorana L.) extracts.Industrial Crops and Products 2013, 43, 827-831,
52. Schmidley, J. Free radicals in central nervous system ischemia. Stroke 1990, 217, 1086-1090,
53. Hertog, M.G.; Feskens, E.J.; Kromhout, D.; Hertog, M.; Hollman, P.; Hertog, M.; Katan, M. Dietary antioxidant flavonoids and risk of coronary heart disease: the Zutphen Elderly Study. The Lancet 1993, 342, 1007-1011,
54. Holiman, P.C.; Hertog, M.G.; Katan, M.B. Analysis and health effects of flavonoids. Food Chemistry 1996, 57, 43-46,
55. Moure, A.; Cruz, J.M.; Franco, D.; omıng e , J.M.; Sineiro, J.; omıng e , H.; José Núñez, M.A.; Parajó, J.C. Natural antioxidants from residual sources. Food Chemistry 2001, 72, 145-171,
56. Halliwell, B. The role of oxygen radicals in human disease, with particular reference to the vascular system.Pathophysiology of Haemostasis & Thrombosis 1993, 23, 118-126,
57. Meyer, A.S.; Heinonen, M.; Frankel, E.N. Antioxidant interactions of catechin, cyanidin, caffeic acid, quercetin, and ellagic acid on human LDL oxidation.Food Chemistry 1998, 61, 71-75,
58. Hunt,E.J.; Lester, C.E.; Lester, E.A.; Tackett, R.L. Effect of St. John's wort on free radical production.Life sciences 2001, 69, 181-190,
How to Cite
Hassan, A., Ullah, I., & Ahmad , W. (2021). Isolation and Evaluation of Antibacterial Potential Test of Plant Carthamus oxycantha. Journal of Tropical Pharmacy and Chemistry, 5(4), 299-308. Retrieved from