Formulation and Evaluation of an Antifungal Ointment Containing Zingiber officinale Roscoe (Zingiberaceae) Essential Oil as Active Principle

Authors

  • Nelly Tagnin Nkuete Centre of Research on Medicinal Plants and Traditional Medicine, Institute of Medical Research and Medicinal Plants studies, Ministry of Scientific Research and Innovation, P.O.Box: 130033 Yaounde-Cameroon
  • Sandra Leila Nnanga Centre of Research on Medicinal Plants and Traditional Medicine, Institute of Medical Research and Medicinal Plants studies, Ministry of Scientific Research and Innovation, P.O.Box: 130033 Yaounde-Cameroon
  • Stephanie Tamdem Guetchueng Institute of medical research and medicinal plants studies
  • Nga Nnanga Centre of Research on Medicinal Plants and Traditional Medicine, Institute of Medical Research and Medicinal Plants studies, Ministry of Scientific Research and Innovation, P.O.Box: 130033 Yaounde-Cameroon
  • Joseph Ngoupayo Department of Pharmacognosy and Pharmaceutical Chemistry, Faculty of Medicine and Biomedical Sciences, University of Yaoundé I, Yaoundé, Cameroon

DOI:

https://doi.org/10.25026/jtpc.v6i1.404

Keywords:

Essential oil; Zingiber officinale; antifungal; ointments

Abstract

The increase incidence of fungal infections, the resistance of fungal strains to existing drugs and the toxicity assimilated to long term usage of antifungal drugs as prompted the search of new alternative from natural source. In this study we have evaluated the antifungal properties of the essential oil of the rhizome of Z. officinale, a plant known for its various biological properties and used in food industry as spice. An antifungal ointment with Z. officinale essential oil as active principle was also formulated and its antifungal properties evaluated. GC/MS analysis identified Zingiberene (22.36%), 1.8-cineole (15.54%), geranial (11.91%), camphene (11.09%) and geraniol (8.75%) as the main constituents of Z. officinale essential oil obtained by hydrodistillation. The oil showed a significant antifungal activity against clinical isolate of Candida krusei with MIC 800 ppm by microdilution method. The formulated ointment containing 10 % glycerol, 60 % vaseline, 8 % hydrosol, 20% lanolin and 2% Zingiber officinale essential oil (Formule E1) was found to follow pharmaceutical standards according to European Pharmacopeia and also showed good antifungal properties

Downloads

Download data is not yet available.

References

Bitar D, Che D, Ancelle T, Fuhrman C, 2013. Bulletin épidémiologique hebdomadaire. 107–108.

Develoux M, Bretagne S, 2005. Candidoses et levuroses diverses. EMC - Maladies Infectieuses, 2(3), 1–15. https://doi.org/10.1016/s1166-8598(05)26088-x

Barelle C J, Richard M L, Gaillardin C, Gow N A R., Brown A J P, 2006. Candida albicans VAC8 is required for vacuolar inheritance and normal hyphal branching. Eukaryotic Cell, 5(2), 359–367. https://doi.org/10.1128/EC.5.2.359-367.2006

Andriatsihoarana S.M., 2010. Contribution a l ’ etude de l ’ huile Andriatsihoarana Sitraka Mbolanirina Andriatsihoarana Sitraka Mbolanirina.

Sudberry P E, 2001. The germ tubes of Candida albicans hyphae and pseudohyphae show different patterns of septin ring localization. Molecular Microbiology, 41(1), 19–31. https://doi.org/10.1046/j.1365-2958.2001.02459.x

Beristain-Bauza S D C, Hernández-Carranza P, Cid-Pérez TS, Ávila-Sosa R, Ruiz-López I I, Ochoa-Velasco C E, 2019. Antimicrobial activity of ginger (Zingiber officinale) and its application in food products. Food Reviews International, 35(5), 407-426.

Zick S M, Djuric Z, Ruffin M T, Litzinger A J, Normolle D P, Alrawi S, Feng M R, Brenner D E, 2008. Pharmaconkinetics of 6-Gingerol, 8-Gingerol, 10-Gingerol, and 6-Shogaol and Conjugate Metabolites in Healthy Human Subjects. Cancer Epidemil. Biomarkers Prev 17, 1930–1936. DOI: 10.1158/1055-9965.EPI-07-2934

Massiot G, 1994. Pharmacognosie: Phytochimie, Plantes Médicinales:?: By J. Bruneton, 2nd Edn, Tec Doc, Paris, 1993. 928pp. 78OF ISBN 2-85206-911-3. Phytochemistry, 36, 258.

Clinical and Laboratory Standards Institute (CLSI), 2008. Reference method for broth dilution. Reference Method for Broth Dilution Antifungal Susceptibility Testing of Yeasts. Approved Standard, 3th Ed., 28(14), 0–13. https://clsi.org/media/1461/m27a3_sample.pdf

Biyiti F, Tamze V, Nnanga N, 2009. Formulation d’une pommade antibactérienne à base d’un extrait éthanolique des écorces du tronc de tabernaemontana crassa benth. Centre de Recherche En Plantes Médicinales et En Médecine Traditionnelle de l’Institut de Recherche Médicale et d’Etude Des Plantes Médicinales (IMPM), 15(January), 1–15.

Bates R G, 1973. Determination of pH; theory and practice. Wiley.

Abdulfattah S, 1991. Stabilité et conditionnement de problèmes de points-selles et loi des grands nombres en analyse épi/hypographique. http://www.theses.fr/1991MON20282

Europe Conseil, 2007. Pharmacopée européenne 6ème Édition (Issue vol.~1~à~2). Conseil de l’Europe. https://books.google.cm/books?id=W-EOJQAACAAJ

Akakpo-akue M, 2009. Évaluation de l’activite antifongique et essai clinique d’une creme formulee a base de l’extrait x12 de Terminalia catappa, sur quelques mycoses superficielles. 13, 175–190.

WHO, 2007. WHO guidelines for assessing quality of herbal medicines with reference to contaminants and residues.

Bouin A S, Wierer M, 2014. Quality standards of the European Pharmacopoeia. Journal of Ethnopharmacology, 158(PART B), 454–457. https://doi.org/10.1016/j.jep.2014.07.020

Bouin A S, Wierer M, 2014. Quality standards of the European Pharmacopoeia. Journal of Ethnopharmacology, 158(PART B), 454–457. https://doi.org/10.1016/j.jep.2014.07.020

Bouin A S, Wierer M, 2014. Quality standards of the European Pharmacopoeia. Journal of Ethnopharmacology, 158(PART B), 454–457. https://doi.org/10.1016/j.jep.2014.07.020

Pozzatti P, Loreto É S, Lopes P G M, Athayde M L, Santurio J M, Alves S H, 2010. Comparison of the susceptibilities of clinical isolates of Candida albicans and Candida dubliniensis to essential oils. Mycoses, 53(1), 12–15. https://doi.org/10.1111/j.1439-0507.2008.01643.x

Sivasothy Y, Chong W K, Hamid A, Eldeen I M, Sulaiman S F, Awang K, 2011. Essential oils of Zingiber officinale var. rubrum Theilade and their antibacterial activities. Food Chemistry, 124(2), 514–517. https://doi.org/10.1016/j.foodchem.2010.06.062

Zouari N, 2013. Essential Oils Chemotypes: A Less Known Side. Medicinal & Aromatic Plants, 02(02). https://doi.org/10.4172/2167-0412.1000e145

Goetz P, Ghedira K, 2012. Introduction à la phytothérapie anti-infectieuse. In Phytothérapie anti-infectieuse (pp. 3–14). Springer Paris. https://doi.org/10.1007/978-2-8178-0058-5_1

Leite M C A, De Brito Bezerra A P, De Sousa J P, Guerra F Q S, De Oliveira Lima E, 2014. Evaluation of antifungal activity and mechanism of action of citral against Candida albicans. Evidence-Based Complementary and Alternative Medicine https://doi.org/10.1155/2014/378280

Kalafat S, Madjbar K, 2017. Extraction d'huile essentielle, application a la formulation d'une pommade antiinflammatoire. Memoire en Genie des Procedes. Universite de BIDA 1. 2017

Downloads

Published

2022-06-30

How to Cite

Nkuete, N. T., Nnanga, S. L., Guetchueng, S. T., Nnanga, N., & Ngoupayo, J. (2022). Formulation and Evaluation of an Antifungal Ointment Containing Zingiber officinale Roscoe (Zingiberaceae) Essential Oil as Active Principle. Journal of Tropical Pharmacy and Chemistry, 6(1), 38–48. https://doi.org/10.25026/jtpc.v6i1.404